| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIS3L2 |
| Uniprot No | Q8IYB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-619aa |
| 氨基酸序列 | MSHPDYRMNLRSLGTHRGVSAVAGPHDIGASPGDKKSKNRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESDIPEELCGHHLPQQSLKSYNDSPDVIVEAQFDGSDSEDGHGITQNVLVDGVKKLSVCVSEKGREDGDAPVTKDETTCISQDTRALSEKSLQRSAKVVYILEKKHSRAATGFLKLLADKNSELFRKYALFSPSDHRVPRIYVPLKDCPQDFVARPKDYANTLFICRIVDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIPPEEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTIIRSCTKLSYEHAQSMIESPTEKIPAKELPPISPEHSSEEVHQAVLNLHGIAKQLRQQRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRESNKPCCAGTPRPKQGCSVTWWNSATRWGCPWTSAPQEPSIKA |
| 分子量 | 95.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4条关于重组人DIS3L2蛋白的参考文献概览：
1. **文献名称**：*Structural insights into the RNA exosome complex revealed by DIS3L2's substrate recognition mechanism*
**作者**：Thomas, J. et al. (2019)
**摘要**：该研究解析了重组人DIS3L2蛋白的晶体结构，揭示了其通过PIN结构域选择性识别并降解特定尿苷化RNA底物的分子机制，为DIS3L2在RNA质量控制中的作用提供结构基础。
2. **文献名称**：*DIS3L2 mutations impair RNA degradation and drive Wilms tumor development*
**作者**：Suzuki, A. et al. (2020)
**摘要**：研究利用重组DIS3L2蛋白进行功能实验，发现其突变导致RNA降解能力丧失，并与Wilms肿瘤发生相关，强调了DIS3L2在维持细胞RNA稳态中的关键性。
3. **文献名称**：*Expression and biochemical characterization of recombinant human DIS3L2 for functional studies*
**作者**：Borgese, L. et al. (2021)
**摘要**：开发了一种高效的大肠杆菌表达系统，用于制备高纯度重组DIS3L2蛋白，并通过酶动力学实验验证其对多聚尿苷化RNA底物的特异性降解活性。
4. **文献名称**：*DIS3L2 in human disease: From ribosome surveillance to therapeutic potential*(综述)
**作者**：Mittal, R. (2022)
**摘要**：综述总结了DIS3L2在RNA代谢和疾病中的多重功能，包括重组蛋白在探索其分子机制及作为癌症治疗靶点中的研究进展。
**注**：以上为模拟文献，实际引用需以具体检索结果为准。建议通过PubMed或Web of Science以关键词“recombinant human DIS3L2”、“DIS3L2 protein function”进一步筛选近期研究。
Recombinant human DIS3L2 is a genetically engineered form of the DIS3L2 exoribonuclease, a key player in RNA metabolism and quality control. DIS3L2 belongs to the RNase II/R family and functions as a 3'-5' RNA exonuclease. It is structurally characterized by a PIN domain for catalysis and a cold shock domain (CSD) for RNA binding. Unlike its paralogs DIS3 and DIS3L1. which associate with the nuclear exosome, DIS3L2 operates independently in the cytoplasm. It degrades specific RNA substrates, including uridylated mRNAs, misfolded non-coding RNAs, and certain viral RNAs, as part of the cytoplasmic RNA surveillance system.
The recombinant protein is widely used to study RNA decay mechanisms, substrate specificity, and interactions with cofactors like LSM1-7 and SKI complexes. DIS3L2 has medical relevance due to its link to Perlman syndrome and myelodysplastic syndromes, where mutations in DIS3L2 or its regulator TRIM71 disrupt RNA homeostasis, potentially promoting oncogenesis. Its role in mitochondrial RNA processing and retrograde signaling further highlights its biological importance. Recombinant DIS3L2 production (often via E. coli or insect cell systems) enables biochemical assays, structural analysis, and drug discovery targeting RNA degradation pathways implicated in cancers and genetic disorders. Research continues to explore its therapeutic potential in diseases marked by RNA dysregulation.
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