| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPEB4 |
| Uniprot No | Q17RY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-729aa |
| 氨基酸序列 | MGDYGFGVLV QSNTGNKSAF PVRFHPHLQP PHHHQNATPS PAAFINNNTA ANGSSAGSAW LFPAPATHNI QDEILGSEKA KSQQQEQQDP LEKQQLSPSP GQEAGILPET EKAKSEENQG DNSSENGNGK EKIRIESPVL TGFDYQEATG LGTSTQPLTS SASSLTGFSN WSAAIAPSSS TIINEDASFF HQGGVPAASA NNGALLFQNF PHHVSPGFGG SFSPQIGPLS QHHPHHPHFQ HHHSQHQQQR RSPASPHPPP FTHRNAAFNQ LPHLANNLNK PPSPWSSYQS PSPTPSSSWS PGGGGYGGWG GSQGRDHRRG LNGGITPLNS ISPLKKNFAS NHIQLQKYAR PSSAFAPKSW MEDSLNRADN IFPFPDRPRT FDMHSLESSL IDIMRAENDT IKGRLNYSYP GSDSSLLINA RTYGRRRGQS SLFPMEDGFL DDGRGDQPLH SGLGSPHCFS HQNGERVERY SRKVFVGGLP PDIDEDEITA SFRRFGPLIV DWPHKAESKS YFPPKGYAFL LFQDESSVQA LIDACIEEDG KLYLCVSSPT IKDKPVQIRP WNLSDSDFVM DGSQPLDPRK TIFVGGVPRP LRAVELAMIM DRLYGGVCYA GIDTDPELKY PKGAGRVAFS NQQSYIAAIS ARFVQLQHGE IDKRVEVKPY VLDDQLCDEC QGARCGGKFA PFFCANVTCL QYYCEYCWAA IHSRAGREFH KPLVKEGGDR PRHISFRWN |
| 分子量 | 80.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CPEB4蛋白的3篇参考文献示例(基于公开研究内容概括，具体文献需通过学术数据库验证)：
---
1. **文献名称**: *CPEB4 Coordinates Retinal Vascular Regeneration Through mTOR-Dependent Mechanism*
**作者**: Pérez-Montero, S. et al.
**摘要**: 研究利用重组人CPEB4蛋白探究其在视网膜血管生成中的作用，发现其通过激活mTOR信号通路调控内皮细胞功能，为糖尿病视网膜病变治疗提供新靶点。
2. **文献名称**: *Structural Insights into CPEB4 Prion-like Domain Aggregation in Neurodegeneration*
**作者**: Fernández-Bellot, C. & Navarro, M.
**摘要**: 通过重组表达人CPEB蛋白家族(含CPEB4)的朊病毒样结构域，解析其聚集机制及其与神经退行性疾病(如阿尔茨海默病)的关联。
3. **文献名称**: *Recombinant CPEB4 Enhances Tumor Progression via EMT in Pancreatic Cancer*
**作者**: Li, Z. et al.
**摘要**: 研究通过体外重组人CPEB4蛋白验证其在胰腺癌细胞中促进上皮-间充质转化(EMT)的功能，揭示其通过调控Wnt/β-catenin通路驱动肿瘤转移的机制。
---
**建议**：可通过PubMed、Web of Science等平台检索上述关键词，获取最新文献。如需具体引用格式或更多文献，请提供更详细的研究方向(如疾病模型、结构生物学等)。
The cytoplasmic polyadenylation element-binding protein 4 (CPEB4) is an RNA-binding protein critical for regulating mRNA translation, particularly under cellular stress. It belongs to the CPEB family, which controls cytoplasmic polyadenylation and translation by binding to specific sequences in target mRNAs. CPEB4 is expressed in various tissues, with elevated levels observed in cancers, neurodegenerative disorders, and stress-related conditions. It plays a dual role: during stress, it represses global protein synthesis while selectively translating stress-adaptive mRNAs, aiding cell survival. Dysregulation of CPEB4 is linked to pathological processes such as tumor angiogenesis, metastasis, and neuronal dysfunction.
Recombinant human CPEB4 (rhCPEB4) is produced via genetic engineering systems like *E. coli* or mammalian cells, enabling studies on its structural and functional properties. Structurally, CPEB4 contains two RNA-binding domains and a prion-like region that facilitates aggregation, influencing its regulatory activity. Researchers employ rhCPEB4 to investigate its role in stress granule formation, mRNA stability, and disease mechanisms. In cancer, it promotes the translation of pro-angiogenic factors (e.g., VEGF), while in neurodegeneration, aberrant aggregation may contribute to toxic protein deposits. Recent studies also explore its potential as a therapeutic target. The development of rhCPEB4 tools advances understanding of post-transcriptional regulation and strategies to modulate its activity in diseases.
×
