| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX8A |
| Uniprot No | P10176 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-69aa |
| 氨基酸序列 | MSVLTPLLLRGLTGSARRLPVPRAKIHSLPPEGKLGIMELAVGLTSCFVTFLLPAGWILSHLETYRRPE |
| 分子量 | 34 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是围绕重组人COX8A蛋白研究的模拟参考文献示例(基于常见研究方向的合理推测)：
1. **文献名称**：*Expression and purification of recombinant human COX8A in Escherichia coli for structural studies*
**作者**：Zhang L., et al.
**摘要**：报道了通过大肠杆菌表达系统高效表达重组人COX8A蛋白，并利用亲和层析技术纯化获得高纯度蛋白，为后续结构解析奠定基础。
2. **文献名称**：*Structural insights into the COX4/COX8A heterodimer in mitochondrial cytochrome c oxidase assembly*
**作者**：Thompson R., et al.
**摘要**：通过冷冻电镜技术解析了COX8A与COX4亚基的复合物结构，揭示了COX8A在线粒体氧化磷酸化复合体组装中的关键作用。
3. **文献名称**：*Tissue-specific expression and metabolic regulation of COX8A in human skeletal muscle*
**作者**：Garcia M., et al.
**摘要**：研究显示重组人COX8A在哺乳动物细胞中的表达受能量代谢信号调控，且其在骨骼肌中的异常表达可能与胰岛素抵抗相关。
4. **文献名称**：*COX8A mutations impair mitochondrial function and cause neuromuscular disorders*
**作者**：Chen J., et al.
**摘要**：发现COX8A基因突变导致蛋白功能缺陷，通过重组蛋白功能回补实验验证其与线粒体能量代谢障碍及神经肌肉疾病的相关性。
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**说明**：上述内容为模拟文献，实际引用需查询PubMed、Web of Science等数据库，并核对原文信息。
Recombinant human COX8A (cytochrome c oxidase subunit 8A) is a nuclear-encoded subunit of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain (ETC) responsible for oxygen reduction and ATP synthesis. As part of Complex IV, COX8A contributes to the assembly, stability, and catalytic efficiency of the holoenzyme. Unlike larger catalytic subunits, COX8A is a small, peripheral component with tissue-specific isoforms (e.g., COX8A in most tissues and COX8B in lungs). Its precise functional role remains less characterized but is implicated in modulating COX activity under varying physiological conditions.
The recombinant form of COX8A is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for structural, functional, or interaction studies. Purification often involves affinity tags (His-tag, GST-tag) followed by chromatography. Recombinant COX8A enables researchers to investigate molecular mechanisms of mitochondrial disorders, including COX deficiencies linked to neurodegenerative diseases, metabolic syndromes, and cancer. It also serves as a tool to map protein-protein interactions within the ETC, study effects of mutations, or develop targeted therapies. Notably, post-translational modifications (e.g., phosphorylation) and isoform-specific roles of COX8A in cellular energy regulation remain active areas of research, highlighting its broader relevance in mitochondrial biology and disease pathology.
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