| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CORO1C |
| Uniprot No | Q9ULV4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474aa |
| 氨基酸序列 | MRRVVRQSKFRHVFGQAVKNDQCYDDIRVSRVTWDSSFCAVNPRFVAIIIEASGGGAFLVLPLHKTGRIDKSYPTVCGHTGPVLDIDWCPHNDQVIASGSEDCTVMVWQIPENGLTLSLTEPVVILEGHSKRVGIVAWHPTARNVLLSAGCDNAIIIWNVGTGEALINLDDMHSDMIYNVSWNRNGSLICTASKDKKVRVIDPRKQEIVAEKEKAHEGARPMRAIFLADGNVFTTGFSRMSERQLALWNPKNMQEPIALHEMDTSNGVLLPFYDPDTSIIYLCGKGDSSIRYFEITDESPYVHYLNTFSSKEPQRGMGYMPKRGLDVNKCEIARFFKLHERKCEPIIMTVPRKSDLFQDDLYPDTAGPEAALEAEEWFEGKNADPILISLKHGYIPGKNRDLKVVKKNILDSKPTANKKCDLISIPKKTTDTASVQNEAKLDEILKEIKSIKDTICNQDERISKLEQQMAKIAA |
| 分子量 | 79.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人CORO1C蛋白的参考文献摘要概括：
1. **标题**：*Structural characterization of human CORO1C reveals dimerization through coiled-coil domains*
**作者**：Zhang et al.
**摘要**：该研究解析了重组人CORO1C蛋白的晶体结构，发现其通过卷曲螺旋结构域形成二聚体，并揭示了与肌动蛋白结合的潜在位点，为理解其在细胞骨架重塑中的作用提供了结构基础。
2. **标题**：*Recombinant CORO1C promotes breast cancer cell migration via Arp2/3-mediated actin polymerization*
**作者**：Li & Chen
**摘要**：通过体外实验表明，重组CORO1C蛋白通过激活Arp2/3复合体增强肌动蛋白聚合，促进乳腺癌细胞迁移，提示其在肿瘤转移中的调控机制。
3. **标题**：*Optimization of recombinant CORO1C expression in E. coli for functional studies*
**作者**：Müller et al.
**摘要**：研究系统优化了在大肠杆菌中表达和纯化重组CORO1C的条件，采用亲和层析和尺寸排阻色谱法获得高纯度蛋白，为后续生化和药物筛选实验奠定基础。
注：以上内容基于领域内常见研究方向构造，实际文献需通过学术数据库查询确认。
**Background of Recombinant Human CORO1C Protein**
Coronin 1C (CORO1C), a member of the coronin protein family, is an actin-binding protein critical for regulating cytoskeletal dynamics, cell migration, and membrane trafficking. Primarily expressed in immune cells, fibroblasts, and cancer cells, CORO1C interacts with actin filaments and the Arp2/3 complex to modulate actin polymerization, influencing cell shape, motility, and intracellular transport. Its N-terminal coiled-coil domain and C-terminal WD40 repeats mediate protein-protein interactions, enabling roles in phagocytosis, vesicle formation, and immune response modulation.
Studies link CORO1C to pathological processes, including cancer metastasis, where it promotes invasive cell behavior by enhancing invadopodia formation and extracellular matrix degradation. Dysregulation of CORO1C is also implicated in immune disorders and neurodegenerative diseases. Recombinant human CORO1C, produced via expression systems like *E. coli* or mammalian cells, retains functional domains for *in vitro* studies, enabling mechanistic exploration of its biological roles. Its applications span cancer research, immunology, and drug development, with potential as a therapeutic target or diagnostic biomarker. Research continues to unravel its precise signaling pathways and disease-specific contributions.
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