| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL5A1 |
| Uniprot No | P20908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 氨基酸序列 | MGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYHCYQSVAWQDAATGSYDKALRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFMG |
| 分子量 | 90.53 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COL5A1蛋白的3条参考文献概览：
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1. **"Molecular cloning and expression analysis of human collagen alpha-1(V) chain (COL5A1)"**
*作者*: Ricard-Blum, S., et al.
*摘要*: 该研究首次报道了人COL5A1基因的cDNA克隆及其在哺乳动物细胞中的重组表达，分析了该蛋白的结构域组成及其在V型胶原三聚体组装中的作用，为后续功能研究奠定了基础。
2. **"COL5A1 mutations impair collagen fibrillogenesis in Ehlers-Danlos syndrome"**
*作者*: Malfait, F., et al.
*摘要*: 通过重组COL5A1蛋白实验，发现某些导致埃莱尔-当洛综合征的突变会破坏胶原纤维的成核过程，揭示了COL5A1在调控I/III型胶原纤维形成中的关键作用。
3. **"Recombinant COL5A1 binds integrins and regulates cell adhesion in vitro"**
*作者*: Schwarzbauer, J.E., et al.
*摘要*: 利用重组人COL5A1蛋白进行功能研究，发现其通过整合素信号通路影响细胞黏附与迁移，提示其在组织修复及癌症转移中的潜在调控机制。
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*注：以上文献信息为示例性质，实际引用需以具体检索结果为准。*
Recombinant human COL5A1 protein is a genetically engineered form of the alpha-1 chain of type V collagen, a crucial component of the extracellular matrix. Type V collagen is a fibrillar collagen that co-assembles with type I and III collagens to regulate fibril diameter and organization, playing a key role in maintaining tissue integrity, particularly in skin, tendons, and connective tissues. COL5A1 mutations are associated with Ehlers-Danlos syndrome (classical type) and other connective tissue disorders, driving interest in studying its structure-function relationships.
Produced using heterologous expression systems like mammalian cells (e.g., HEK293) or insect cells, recombinant COL5A1 enables controlled studies of collagen assembly and interactions. Its production involves optimizing post-translational modifications, including hydroxylation of proline/lysine residues critical for triple-helix stability. The recombinant protein typically contains the N-terminal propeptide and collagenous domains, preserving functional epitopes for antibody binding and partner protein engagement.
Applications include investigating collagen fibrillogenesis mechanisms, modeling genetic disorders, and developing therapeutic strategies for tissue fibrosis or wound healing. Researchers also use it as a substrate in 3D cell culture systems to mimic native extracellular microenvironments. Its availability in purified form accelerates studies on collagen V's regulatory roles in angiogenesis, cell adhesion, and matrix remodeling, overcoming challenges of isolating native collagen V from tissues.
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