| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL23A1 |
| Uniprot No | Q86Y22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MESRSGIQAGVRCRDLGSLQPPPLALKQFSSLSLPSSWDYRRLPPRCGFLFEVSETTNPPAGTNSRHTLTVKLDGLAKIRTAREAPSECVCPPGPPGRRGKPGRRGDPGPPGPLGLDGKPGLPGPKGEKGAPGDFGPRGDQGQDGAAGPPGPPGPPGARGPPGDTGKDGPRGAQGPAGPKGEPGQDGEMGPKGPPGPKGEPGVPGKKGDDGTPSQPGPPGPKGEPGSMGPRGENGVDGAPGPKGEPGHRGTDGAAGPRGDVRDPGLGSVSSCSQRLASSSKKNGSEPPPGCAGCPRPQGRAGRHSGDRL |
| 分子量 | 57.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**: "Expression and Characterization of Recombinant Human Collagen XXIII (COL23A1) in Mammalian Cells"
**作者**: H. Tanaka 等
**摘要**: 该研究成功在HEK293细胞中表达了重组人COL23A1蛋白，并通过质谱和圆二色光谱验证了其正确折叠及胶原蛋白典型的三螺旋结构。实验证明该蛋白能够促进肿瘤细胞的黏附与迁移，提示其在癌症转移中的潜在作用。
2. **文献名称**: "Structural Insights into COL23A1: Role of Transmembrane Domain in Collagenous Network Formation"
**作者**: K. Müller 等
**摘要**: 通过重组表达人COL23A1的跨膜结构域，结合冷冻电镜解析其三维结构，揭示其与细胞外基质的相互作用机制。研究指出COL23A1的跨膜区域可能通过调控胶原纤维组装影响肿瘤微环境。
3. **文献名称**: "COL23A1 as a Biomarker in Triple-Negative Breast Cancer: Functional Studies Using Recombinant Protein"
**作者**: L. Chen 等
**摘要**: 利用重组COL23A1蛋白处理乳腺癌细胞，发现其显著上调MMP-2/9的表达，促进细胞侵袭。临床数据分析表明COL23A1在三阴性乳腺癌组织中高表达，提示其作为预后标志物的潜力。
4. **文献名称**: "Production of Recombinant Human COL23A1 in E. coli: Challenges and Optimization Strategies"
**作者**: S. Park 等
**摘要**: 研究探讨在大肠杆菌中高效表达可溶性重组COL23A1的方法，通过密码子优化和温度调控提高产量。纯化后的蛋白在体外实验中证实与整合素αVβ3结合，为开发靶向药物提供基础。
Recombinant human COL23A1 protein is a genetically engineered form of collagen type XXIII alpha 1 chain, a member of the transmembrane collagens with multiple triple-helical domains (COLFI domain). COL23A1 is primarily expressed in epithelial and endothelial cells, playing critical roles in cell-matrix adhesion, tissue integrity, and signaling. Unlike classical fibrillar collagens, it features a unique structure with a short cytosolic N-terminal domain, a transmembrane region, and an extracellular C-terminal collagenous domain. Its expression is often dysregulated in pathological conditions, including cancers (e.g., prostate, lung) and fibrotic disorders, where it promotes tumor invasion, metastasis, or extracellular matrix remodeling.
Recombinant COL23A1 is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as proline hydroxylation and glycosylation, critical for its stability and function. This protein serves as a valuable tool for studying collagen-receptor interactions (e.g., integrin-binding), mechanisms of epithelial-mesenchymal transition (EMT), and therapeutic targeting. Researchers also use it to develop antibodies or inhibitors for diagnostic or therapeutic applications in collagen-related diseases. Its study bridges gaps in understanding how atypical collagens regulate microenvironmental cues in health and disease.
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