| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL4A3BP |
| Uniprot No | Q9Y5P4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-598aa |
| 氨基酸序列 | MSDNQSWNSSGSEEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNALSYYKSEDETEYGCRGSICLSKAVITPHDFDECRFDISVNDSVWYLRAQDPDHRQQWIDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDACADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHSTNGNKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREDSWQKRLDKETEKKRRTEEAYKNAMTELKKKSHFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPTSLPSGDAFSSVGTHRFVQKVEEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSVIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINVAMICQTLVSPPEGNQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF |
| 分子量 | 94.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人COL4A3BP蛋白的虚构参考文献示例(注：实际文献需查阅科研数据库)：
1. **文献名称**: "Expression and Purification of Recombinant Human COL4A3BP in E. coli and Its Interaction with Collagen IV"
**作者**: Zhang L., et al.
**摘要**: 本研究利用大肠杆菌表达系统成功表达重组人COL4A3BP蛋白，通过His标签亲和层析纯化后证实其与IV型胶原蛋白的α3链特异性结合，揭示其在基底膜组装中的潜在作用。
2. **文献名称**: "Functional Analysis of COL4A3BP in Tumor Angiogenesis: A Recombinant Protein Approach"
**作者**: Tanaka K., et al.
**摘要**: 通过哺乳动物细胞表达重组人COL4A3BP，发现其通过抑制内皮细胞迁移和管腔形成降低肿瘤血管生成，提示其在抗肿瘤治疗中的应用前景。
3. **文献名称**: "Structural Insights into COL4A3BP-CERT Complex by Cryo-EM Using Recombinant Protein"
**作者**: Müller R., et al.
**摘要**: 利用冷冻电镜解析重组人COL4A3BP与CER转移蛋白(CERT)复合物的三维结构，阐明其在鞘脂代谢中的分子机制及转运体功能的结构基础。
(注：以上文献为假设性示例，实际研究中需以真实文献为准。)
Recombinant human COL4A3BP protein, also known as collagen type IV alpha-3 binding protein or STARD10 (StAR-related lipid transfer domain protein 10), is a multifunctional protein involved in extracellular matrix dynamics and intracellular lipid transport. It binds to the α3 chain of type IV collagen, a key structural component of basement membranes, playing roles in tissue integrity and cellular adhesion. Simultaneously, as a member of the StAR-related lipid transfer family, COL4A3BP/STARD10 facilitates ceramide and glycosphingolipid trafficking, influencing lipid metabolism and membrane biogenesis.
This protein is genetically linked to Alport syndrome, a hereditary disorder characterized by kidney dysfunction and hearing loss, due to mutations in type IV collagen genes. Dysregulation of COL4A3BP has also been implicated in cancer progression, diabetes, and cardiovascular diseases, highlighting its broad pathophysiological relevance.
Recombinant COL4A3BP is engineered using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications and functional activity. It serves as a critical tool for studying collagen-lipid interactions, disease mechanisms, and therapeutic targeting. Applications include in vitro binding assays, cell migration studies, and drug screening platforms. Its high purity and bioactivity make it valuable for both basic research and potential clinical developments aimed at modulating extracellular matrix remodeling or lipid-related pathologies.
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