| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDK5RAP1 |
| Uniprot No | Q96SZ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-601aa |
| 氨基酸序列 | MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG ARKDFSSRLA AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR KVYLETYGCQ MNVNDTEIAW SILQKSGYLR TSNLQEADVI LLVTCSIREK AEQTIWNRLH QLKALKTRRP RSRVPLRIGI LGCMAERLKE EILNREKMVD ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP VQTSASATSA FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQVFLPPRPP KVLGLQGLKE VTLLGQNVNS FRDNSEVQFN SAVPTNLSRG FTTNYKTKQG GLRFAHLLDQ VSRVDPEMRI RFTSPHPKDF PDEVLQLIHE RDNICKQIHL PAQSGSSRVL EAMRRGYSRE AYVELVHHIR ESIPGVSLSS DFIAGFCGET EEDHVQTVSL LREVQYNMGF LFAYSMRQKT RAYHRLKDDV PEEVKLRRLE ELITIFREEA TKANQTSVGC TQLVLVEGLS KRSATDLCGR NDGNLKVIFP DAEMEDVNNP GLRVRAQPGD YVLVKITSAS SQTLRGHVLC RTTLRDSSAY C |
| 分子量 | 100 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人CDK5RAP1蛋白的3篇代表性文献(基于公开研究整理，可能存在简化或示例性内容)：
1. **文献名称**：*CDK5RAP1 interacts with CENP-F to regulate mitotic progression and genome stability*
**作者**：Chen H, et al.
**摘要**：研究发现CDK5RAP1通过与CENP-F相互作用，参与调控有丝分裂中染色体的正确分离和基因组稳定性。实验显示，重组CDK5RAP1蛋白的缺失导致细胞周期阻滞和微管组织异常。
2. **文献名称**：*CDK5RAP1 regulates neural progenitor differentiation by modulating centrosomal microtubule nucleation*
**作者**：Tang W, et al.
**摘要**：本文通过体外重组蛋白实验，证明CDK5RAP1通过调节中心体微管成核能力，影响神经前体细胞的分化和神经元迁移，揭示其在脑发育中的关键作用。
3. **文献名称**：*Structural and functional analysis of CDK5RAP1 reveals a conserved motif essential for pericentriolar material targeting*
**作者**：Lüders J, et al.
**摘要**：通过重组人CDK5RAP1蛋白的结构解析，鉴定了其靶向中心体周围基质的关键保守基序，并验证该基序对其调控微管组装功能的必要性。
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**说明**：
以上文献为示例性内容，整合了CDK5RAP1在细胞周期、神经发育及结构功能等领域的研究方向。如需具体文献，建议通过PubMed或Google Scholar检索关键词“CDK5RAP1 recombinant protein”或“CDK5RAP1 function”，结合近年研究筛选。例如，真实文献可能包括《Journal of Cell Science》或《Molecular Biology of the Cell》等期刊的相关论文。
CDK5RAP1 (CDK5 Regulatory Subunit Associated Protein 1) is a conserved eukaryotic protein implicated in regulating cell cycle progression and microtubule dynamics. It interacts directly with CDK5R1 (p35), a neuronal activator of cyclin-dependent kinase 5 (CDK5), influencing CDK5’s subcellular localization and activity. Structurally, CDK5RAP1 contains multiple coiled-coil domains and a conserved CM1 motif critical for binding CDK5-p35 complexes. While CDK5 is best known for its role in neuronal development and neurodegenerative disorders, CDK5RAP1's functions extend beyond the nervous system. Studies suggest its involvement in mitotic spindle organization, centrosome maturation, and genomic stability through interactions with γ-tubulin and other microtubule-associated proteins.
Recombinant human CDK5RAP1 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables mechanistic studies of its biochemical properties and disease associations. Dysregulation of CDK5RAP1 has been linked to neurodevelopmental defects, cancer progression, and abnormal cell proliferation, likely due to its dual roles in CDK5 signaling and mitotic fidelity. Current research focuses on its potential as a therapeutic target, particularly in glioblastoma and other cancers with disrupted centrosome amplification pathways. However, its tissue-specific regulatory networks and post-translational modifications remain poorly characterized, highlighting the need for further structural and functional analyses using recombinant protein tools.
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