| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDH4 |
| Uniprot No | P55283 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-916aa |
| 氨基酸序列 | MTAGAGVLLLLLSLSGALRAHNEDLTTRETCKAGFSEDDYTALISQNILEGEKLLQVKFSSCVGTKGTQYETNSMDFKVGADGTVFATRELQVPSEQVAFTVTAWDSQTAEKWDAVVRLLVAQTSSPHSGHKPQKGKKVVALDPSPPPKDTLLPWPQHQNANGLRRRKRDWVIPPINVPENSRGPFPQQLVRIRSDKDNDIPIRYSITGVGADQPPMEVFSIDSMSGRMYVTRPMDREEHASYHLRAHAVDMNGNKVENPIDLYIYVIDMNDNRPEFINQVYNGSVDEGSKPGTYVMTVTANDADDSTTANGMVRYRIVTQTPQSPSQNMFTINSETGDIVTVAAGLDREKVQQYTVIVQATDMEGNLNYGLSNTATAIITVTDVNDNPPEFTASTFAGEVPENRVETVVANLTVMDRDQPHSPNWNAVYRIISGDPSGHFSVRTDPVTNEGMVTVVKAVDYELNRAFMLTVMVSNQAPLASGIQMSFQSTAGVTISIMDINEAPYFPSNHKLIRLEEGVPPGTVLTTFSAVDPDRFMQQAVRYSKLSDPASWLHINATNGQITTAAVLDRESLYTKNNVYEATFLAADNGIPPASGTGTLQIYLIDINDNAPELLPKEAQICEKPNLNAINITAADADVDPNIGPYVFELPFVPAAVRKNWTITRLNGDYAQLSLRILYLEAGMYDVPIIVTDSGNPPLSNTSIIKVKVCPCDDNGDCTTIGAVAAAGLGTGAIVAILICILILLTMVLLFVMWMKRREKERHTKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPEAMGHVPSKAPGVRRVDERPVGAEPQYPIRPMVPHPGDIGDFINEGLRAADNDPTAPPYDSLLVFDYEGSGSTAGSVSSLNSSSSGDQDYDYLNDWGPRFKKLADMYGGGEED |
| 分子量 | 100 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人钙粘蛋白-4(CDH4)的参考文献示例(内容基于已知研究领域，非真实文献)：
1. **标题**：*CDH4 promotes hepatocellular carcinoma invasion via PI3K/AKT signaling*
**作者**：Kawai, H., et al.
**摘要**：研究显示CDH4在肝细胞癌中高表达，通过激活PI3K/AKT通路增强肿瘤细胞侵袭性，并与患者不良预后相关。
2. **标题**：*Structural characterization of recombinant human CDH4 extracellular domains*
**作者**：Suzuki, S.T., et al.
**摘要**：通过重组技术表达并纯化CDH4胞外结构域，利用X射线晶体学解析其三维结构，揭示钙离子依赖性粘附机制的关键结构基序。
3. **标题**：*CDH4 as a prognostic marker in glioblastoma multiforme*
**作者**：Jang, M.H., et al.
**摘要**：在胶质母细胞瘤中，CDH4高表达与肿瘤干细胞特性及耐药性相关，提示其作为潜在治疗靶点。
4. **标题**：*CDH4 regulates axonal guidance during neural development*
**作者**：Okazaki, Y., et al.
**摘要**：体外实验表明，重组CDH4蛋白通过调控神经元细胞间粘附，影响轴突导向和神经网络形成，支持其在神经发育中的作用。
(注：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以“CDH4”或“Cadherin-4”为关键词检索。)
Cadherin-4 (CDH4), also known as R-cadherin, is a transmembrane glycoprotein belonging to the cadherin superfamily, which mediates calcium-dependent cell-cell adhesion. As a classical type II cadherin, CDH4 plays critical roles in tissue morphogenesis, neural development, and cellular signaling. It is characterized by its extracellular cadherin repeats that facilitate homophilic binding and intracellular domains interacting with catenins to link the actin cytoskeleton. CDH4 is prominently expressed during embryonic development, particularly in the nervous system, retina, and kidneys, where it regulates neuronal migration, axon guidance, and tissue patterning.
Dysregulation of CDH4 has been implicated in multiple pathologies. In cancer, CDH4 exhibits context-dependent roles: it can act as a tumor suppressor by maintaining cell adhesion, while paradoxical overexpression in certain malignancies (e.g., lung adenocarcinoma, glioblastoma) correlates with enhanced invasion and metastasis. Epigenetic silencing of CDH4 via promoter hypermethylation has been observed in colorectal and breast cancers. Beyond oncology, altered CDH4 expression is associated with neurodevelopmental disorders and retinal degeneration.
Recombinant CDH4 proteins, generated through mammalian or insect cell systems, enable structural-functional studies and therapeutic exploration. Current research focuses on deciphering its dual roles in tumor progression and developing cadherin-based targeting strategies.
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