| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDCA7 |
| Uniprot No | Q9BWT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 氨基酸序列 | MDARRVPQKDLRVKKNLKKFRYVKLISMETSSSSDDSCDSFASDNFANTRLQSVREGCRTRSQCRHSGPLRVAMKFPARSTRGATNKKAESRQPSENSVTDSNSDSEDESGMNFLEKRALNIKQNKAMLAKLMSELESFPGSFRGRHPLPGSDSQSRRPRRRTFPGVASRRNPERRARPLTRSRSRILGSLDALPMEEEEEEDKYMLVRKRKTVDGYMNEDDLPRSRRSRSSVTLPHIIRPVEEITEEELENVCSNSREKIYNRSLGSTCHQCRQKTIDTKTNCRNPDCWGVRGQFCGPCLRNRYGEEVRDALLDPNWHCPPCRGICNCSFCRQRDGRCATGVLVYLAKYHGFGNVHAYLKSLKQEFEMQA |
| 分子量 | 69 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDCA7蛋白的3-4篇文献示例(文献内容为模拟概括，供参考)：
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1. **文献名称**: *"Cloning and Functional Analysis of Human CDCA7 in MYC-Regulated DNA Replication"*
**作者**: Pounds, S., et al.
**摘要**: 研究通过重组表达人CDCA7蛋白，揭示了其在MYC调控的DNA复制中的关键作用，证明CDCA7与复制起点复合物结合，促进细胞周期S期进程。
2. **文献名称**: *"Recombinant CDCA7 Protein as a Biomarker in Colorectal Cancer Proliferation"*
**作者**: Huh, J.W., et al.
**摘要**: 该文献通过体外表达重组CDCA7蛋白，发现其在结直肠癌细胞中显著过表达，并证明其通过调控Wnt/β-catenin通路促进肿瘤细胞增殖。
3. **文献名称**: *"Structural Characterization and siRNA Interaction of Recombinant Human CDCA7"*
**作者**: Kim, J., & Park, S.
**摘要**: 文章解析了重组CDCA7蛋白的晶体结构，发现其锌指结构域对siRNA介导的基因沉默具有调控作用，可能参与表观遗传修饰机制。
4. **文献名称**: *"CDCA7 Overexpression Enhances Chemoresistance in Ovarian Cancer via Recombinant Protein-Mediated Signaling"*
**作者**: Li, X., et al.
**摘要**: 利用重组CDCA7蛋白实验，发现其在卵巢癌中通过激活PI3K/AKT通路增强癌细胞对顺铂的耐药性，提示其作为治疗靶点的潜力。
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**注**：以上文献信息为模拟概括，实际引用需通过数据库(如PubMed、Web of Science)检索真实文献。如需具体文献，建议使用关键词“recombinant CDCA7 protein”或“CDCA7 cloning”进一步查询。
CDCA7 (Cell Division Cycle Associated Protein 7) is a nuclear protein encoded by the CDCA7 gene in humans, primarily implicated in cell cycle regulation, DNA replication, and chromatin dynamics. It was initially identified as a MYC-responsive gene, with its expression tightly regulated by the MYC oncoprotein, a key driver of cellular proliferation and tumorigenesis. CDCA7 is known to interact with components of the DNA replication machinery, such as the AAA+ ATPase TRESLIN/MTBP, facilitating the initiation of DNA replication during S-phase. Its function is also linked to the maintenance of heterochromatin integrity and chromosome stability, potentially through interactions with chromatin-modifying complexes. Structurally, CDCA7 contains zinc finger-like domains that may mediate protein-DNA or protein-protein interactions. Dysregulation of CDCA7 has been associated with cancer progression, particularly in MYC-driven malignancies like Burkitt’s lymphoma and neuroblastoma, where its overexpression correlates with poor prognosis. Recombinant human CDCA7 protein is commonly produced in vitro (e.g., E. coli or mammalian systems) for functional studies, enabling exploration of its role in replication licensing, cell cycle checkpoints, and oncogenic transformation. Current research focuses on leveraging recombinant CDCA7 to dissect its molecular mechanisms, screen targeted inhibitors, and develop therapeutic strategies against MYC-dependent cancers.
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