| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC2L6 |
| Uniprot No | Q9BWU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-502aa |
| 氨基酸序列 | MDYDFKAKLAAERERVEDLFEYEGCKVGRGTYGHVYKARRKDGKDEKEYALKQIEGTGISMSACREIALLRELKHPNVIALQKVFLSHSDRKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPFHHDQLDRIFSVMGFPADKDWEDIRKMPEYPTLQKDFRRTTYANSSLIKYMEKHKVKPDSKVFLLLQKLLTMDPTKRITSEQALQDPYFQEDPLPTLDVFAGCQIPYPKREFLNEDDPEEKGDKNQQQQQNQHQQPTAPPQQAAAPPQAPPPQQNSTQTNGTAGGAGAGVGGTGAGLQHSQDSSLNQVPPNKKPRLGPSGANSGGPVMPSDYQHSSSRLNYQSSVQGSSQSQSTLGYSSSSQQSSQYHPSHQAHRY |
| 分子量 | 83.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDC2L6蛋白的假设性参考文献示例，涵盖不同研究方向，供参考：
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1. **文献名称**：*Molecular Cloning and Characterization of Human CDC2L6: A Novel Cell Cycle-Regulated Kinase*
**作者**：Smith A, et al.
**摘要**：本研究首次克隆了CDC2L6基因，并成功表达重组蛋白。通过体外激酶活性实验，证实其磷酸化组蛋白H1的能力，提示其在细胞周期G2/M期调控中的潜在作用。
2. **文献名称**：*Functional Analysis of CDC2L6 in Mitotic Progression Using siRNA Knockdown*
**作者**：Johnson B, et al.
**摘要**：利用RNA干扰技术沉默CDC2L6.发现细胞周期在G2期停滞，并伴随纺锤体形成异常。结果表明CDC2L6对有丝分裂进程至关重要。
3. **文献名称**：*CDC2L6 Interacts with Cyclin B1: Implications for Its Role in Apoptosis Regulation*
**作者**：Lee C, et al.
**摘要**：通过免疫共沉淀和质谱分析，鉴定CDC2L6与Cyclin B1的直接结合，并揭示其通过p53通路调控DNA损伤诱导的凋亡，为癌症治疗提供新靶点。
4. **文献名称**：*Overexpression of CDC2L6 in Breast Cancer and Its Correlation with Prognosis*
**作者**：Chen D, et al.
**摘要**：在乳腺癌组织中检测到CDC2L6的异常高表达，且与患者生存率负相关。体外实验显示抑制CDC2L6可减少肿瘤细胞侵袭，提示其临床意义。
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**备注**：以上文献为假设性示例，实际研究中请通过**PubMed、NCBI或Web of Science**等平台检索真实文献(关键词：CDC2L6、CDK11、细胞周期激酶)。若需进一步协助定位文献，请提供更具体的研究方向或基因别名信息。
**Background of Recombinant Human CDC2L6 Protein**
The CDC2L6 protein, also known as cyclin-dependent kinase 11 (CDK11), is a member of the CDK family of serine/threonine kinases, which play pivotal roles in regulating cell cycle progression, transcription, and RNA processing. Encoded by the *CDC2L6* gene located on chromosome 1p36.3. CDK11 exists in two major isoforms (p110 and p58) generated through alternative splicing. It interacts with cyclin L partners to form active complexes essential for mitotic division, particularly in G2/M phase regulation, and has been implicated in apoptosis, neuronal function, and tumorigenesis.
Recombinant human CDC2L6 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and functionality for experimental studies. This engineered protein retains kinase activity, enabling researchers to dissect its interactions, substrate specificity, and regulatory mechanisms *in vitro*. Studies leveraging recombinant CDC2L6 have highlighted its role in cancer progression, as aberrant expression correlates with poor prognosis in multiple cancers. Additionally, it serves as a tool for drug discovery targeting CDK-related pathologies, including neurodegenerative diseases and malignancies. The availability of recombinant CDC2L6 has advanced understanding of its structural dynamics and therapeutic potential within cell cycle and transcriptional networks.
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