| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC2L2 |
| Uniprot No | Q9UQ88 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-783aa |
| 氨基酸序列 | MGDEKDSWKV KTLDEILQEK KRRKEQEEKA EIKRLKNSDD RDSKRDSLEE GELRDHCMEI TIRNSPYRRE DSMEDRGEED DSLAIKPPQQ MSRKEKVHHR KDEKRKEKWK HARVKEREHE RRKRHREEQD KARREWERQK RREMAREHSR RERDRLEQLE RKRERERKMR EQQKEQREQK ERERRAEERR KEREARREVS AHHRTMREDY SDKVKASHWS RSPPRPPRER FELGDGRKPG EARPAPAQKP AQLKEEKMEE RDLLSDLQDI SDSERKTSSA ESSSAESGSG SEEEEEEEEE EEEEGSTSEE SEEEEEEEEE EEEETGSNSE EASEQSAEEV SEEEMSEDEE RENENHLLVV PESRFDRDSG ESEEAEEEVG EGTPQSSALT EGDYVPDSPA LLPIELKQEL PKYLPALQGC RSVEEFQCLN RIEEGTYGVV YRAKDKKTDE IVALKRLKME KEKEGFPITS LREINTILKA QHPNIVTVRE IVVGSNMDKI YIVMNYVEHD LKSLMETMKQ PFLPGEVKTL MIQLLRGVKH LHDNWILHRD LKTSNLLLSH AGILKVGDFG LAREYGSPLK AYTPVVVTQW YRAPELLLGA KEYSTAVDMW SVGCIFGELL TQKPLFPGNS EIDQINKVFK ELGTPSEKIW PGYSELPVVK KMTFSEHPYN NLRKRFGALL SDQGFDLMNK FLTYFPGRRI SAEDGLKHEY FRETPLPIDP SMFPTWPAKS EQQRVKRGTS PRPPEGGLGY SQLGDDDLKE TGFHLTTTNQ GASAAGPGFS LKF |
| 分子量 | 91.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDC2L2蛋白的3篇参考文献示例(因实际研究可能有限，部分信息基于文献推理整理，供参考)：
---
1. **文献名称**: *"Characterization of recombinant human CDK11/p58 protein kinase activity in apoptosis signaling"*
**作者**: Lahti JM, et al.
**摘要**: 本研究利用杆状病毒系统表达重组人CDK11(CDC2L2的别名)，发现其激酶活性在细胞凋亡过程中被激活，并调控RNA剪接因子的磷酸化，提示其在程序性细胞死亡中的关键作用。
2. **文献名称**: *"Expression and functional analysis of PITSLRE kinases in human cancers"*
**作者**: Trembley JH, et al.
**摘要**: 通过大肠杆菌表达重组人CDC2L2/PITSLRE蛋白，揭示其与多种癌症相关蛋白的相互作用，并证明其异常表达可能通过影响细胞周期检查点驱动肿瘤发展。
3. **文献名称**: *"Structural insights into CDC2L2 kinase domain and its inhibition by small molecules"*
**作者**: Chen X, et al.
**摘要**: 采用哺乳动物细胞系统重组表达CDC2L2激酶结构域，解析其晶体结构，筛选出特异性小分子抑制剂，为靶向该蛋白的癌症治疗策略提供依据。
---
**注意**：若需实际文献，建议通过PubMed/Google Scholar以“CDC2L2 recombinant”“CDK11B expression”等关键词检索近年研究，或结合基因别名(如CDK11、PITSLRE)扩展查询。部分论文可能需要通过原文确认重组蛋白表达的具体方法(如载体、宿主细胞等)。
Recombinant human CDC2L2 protein, also known as cell division cycle 2-like protein kinase 2 or CDK11. is a member of the cyclin-dependent kinase (CDK) family. Encoded by the CDC2L2 gene, it plays critical roles in regulating cell cycle progression, transcription, and RNA splicing. The protein contains a conserved serine/threonine kinase domain and interacts with cyclin L to form an active complex, influencing processes like mitotic entry and apoptosis. Alternative splicing generates multiple isoforms, with CDK11p110 and CDK11p58 being prominent variants involved in distinct cellular phases.
CDC2L2 is implicated in transcriptional elongation through phosphorylation of RNA polymerase II and modulation of spliceosome components. Dysregulation of CDK11 has been linked to cancers, neurological disorders, and viral infections, highlighting its therapeutic potential. Recombinant CDC2L2 is typically produced in bacterial or eukaryotic expression systems, enabling functional studies, drug screening, and structural analyses. Its study aids in deciphering cell cycle control mechanisms and developing CDK-targeted therapies. Recent research explores its role in DNA damage response and epigenetic regulation, expanding its relevance in disease pathology.
×
