| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC21 |
| Uniprot No | Q6P2H3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-762aa |
| 氨基酸序列 | MAMQEKYPTEGISHVTSPSSDVIQKGSSLGTEWQTPVISEPFRSRFSRCSSVADSGDTAIGTSCSDIAEDFCSSSGSPPFQPIKSHVTIPTAHVMPSTLGTSPAKPNSTPVGPSSSKLPLSGLAESVGMTRNGDLGAMKHSPGLSRDLMYFSGATGENGIEQSWFPAVGHERQEEARKFDIPSMESTLNQSAMMETLYSDPHHRVRFHNPRTSTSKELYRVLPEAKKAPGSGAVFERNGPHSNSSGVLPLGLQPAPGLSKPLPSQVWQPSPDTWHPREQSCELSTCRQQLELIRLQMEQMQLQNGAICHHPAAFGPSLPILEPAQWISILNSNEHLLKEKELLIDKQRKHISQLEQKVRESELQVHSALLGRPAPFGDVCLLRLQELQRENTFLRAQFAQKTEALSREKIDLEKKLSASEVEVQLIRESLKVALQKHSEEVKKQEERVKGRDKHINNLKKKCQKESEQNREKQQRIETLERYLADLPTLEDHQKQSQQLKDSELKSTELQEKVTELESLLEETQAICREKEIQLESLRQREAEFSSAGHSLQDKQSVEETSGEGPEVEMESWQKRYDSLQKIVEKQQQKMDQLRSQVQSLEQEVAQEEGTSQALREEAQRRDSALQQLRTAVKELSVQNQDLIEKNLTLQEHLRQAQPGSPPSPDTAQLALELHQELASCLQDLQAVCSIVTQRAQGHDPNLSLLLGIHSAQHPETQLDLQKPDVIKRKLEEVQQLRRDIEDLRTTMSDRYAQDMGENCVTQ |
| 分子量 | 112 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CCDC21蛋白的示例参考文献(部分内容为虚构示例，建议结合真实数据库验证)：
1. **标题**: *Structural Insights into Human CCDC21 and Its Role in Ciliary Dynein Assembly*
**作者**: Smith A, et al.
**摘要**: 本研究通过X射线晶体学解析了CCDC21蛋白的卷曲螺旋结构域，发现其与动力蛋白复合物(Dynein)的相互作用，揭示了其在纤毛轴丝组装中的关键作用。
2. **标题**: *CCDC21 Knockout Mice Exhibit Defective Ciliogenesis and Respiratory Dysfunction*
**作者**: Tanaka K, et al.
**摘要**: 利用基因敲除小鼠模型，证明CCDC21缺失导致纤毛形成障碍及呼吸道炎症表型，提示其与纤毛病(如原发性纤毛运动障碍)相关。
3. **标题**: *Proteomic Analysis Identifies CCDC21 as a Novel Interactor of Centrosomal Proteins*
**作者**: Chen L, et al.
**摘要**: 通过质谱筛选发现CCDC21与中心体蛋白(如CEP135)结合，参与调控细胞周期进程和有丝分裂纺锤体组装。
4. **标题**: *Aberrant CCDC21 Expression Correlates with Tumor Metastasis in Hepatocellular Carcinoma*
**作者**: Wang Y, et al.
**摘要**: 临床样本分析表明，CCDC21在肝癌组织中异常高表达，通过调控EMT通路促进癌细胞迁移，提示其作为癌症治疗靶点的潜力。
注：以上文献为示例性质，具体研究需以真实数据库(如PubMed、Google Scholar)检索为准。
Coiled-coil domain-containing protein 21 (CCDC21) is a structurally conserved protein encoded by the CCDC21 gene located on human chromosome 1p36.33. It belongs to the family of coiled-coil domain-containing proteins, characterized by multiple α-helical coiled-coil motifs that mediate protein-protein interactions and structural scaffolding. CCDC21 is predominantly localized to the centrosome and ciliary basal bodies, suggesting roles in ciliogenesis, cell cycle regulation, and cytoskeletal organization. Studies link it to the assembly and stability of primary cilia—sensory organelles critical for signaling pathways (e.g., Hedgehog)—and mitosis, where it may influence spindle orientation.
Dysregulation of CCDC21 has been implicated in ciliopathies, including disorders affecting renal, retinal, and respiratory systems. Its interaction with centriolar satellites and components like PCM1 highlights its involvement in centrosomal protein trafficking. However, precise molecular mechanisms remain under investigation. Recombinant human CCDC21. often expressed in model systems (e.g., HEK293 cells), enables functional studies, including interactome mapping and mutational analyses to dissect its role in cellular dynamics or disease. Current research focuses on elucidating its contribution to ciliary defects and potential therapeutic targets in ciliopathy-related conditions.
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