| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C8orf80 |
| Uniprot No | Q68CJ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-796aa |
| 氨基酸序列 | MAETKDVFGQ EPHPVEDDLY KERTRKRRKS DRDQRFRAFP SMEQSALKEY EKLESRTRRV LSNTYQKLIQ SVFLDDSIPN GVKYLINRLL ALIEKPTVDP IYIALFGSTG AGKSSLINAI IQQAMFLPVS GESICTSCIV QVSSGCCVQY EAKIHLLSDQ EWREELKNLT KLLHRTEELS REEADAWNRD EAVEEATWKL QMIYGNGAES KNYEELLRAK PKRKIPTSRV ITLKAEEAEE LSIKLDPYIR TQRRDWDGEA AEMRIWPLIK HVEVTLPKSD LIPEGVVLVD IPGTGDFNSK RDEMWKKTID KCSVIWVISD IERVSGGQAH EDLLNESIKA CQRGFCRDVA LVVTKMDKLH LPEYLRERKA GNQAIQSQRE AVLERNEMIK LQRTRILKEK LKRKLPADFK VLEASDLVYT VSAQEYWQQA LLTEEETEIP KLREYIRKSL LDKKKRTVTK YVTEAFGLLL LTDSFNSTQN LPNEHLHMSV LRRFAEEKVE LLEKAIAQCF ACMEQPLQEG VRTARTSYRC ILRACLVRSK GNQGFHQTLK AVCLKNGIYA SRTLARIDLN EALTQPVYDQ IDPVFGSIFR TGKPTGSALM PHIDAFKQSL QEKMTEIGIR SGWKYDSCKK NFLIQEISAI LGGLEDHILR RKRRIYESLT ASVQSDLKLC YEEAAQITGK KACERMKDAI RRGVDRQVAE GMFERAQERM QHQFQQLKTG IVEKVKGSIT TMLALASSQG DGLYKELADV GSEYKEMEKL HRSLREVAEN ARLRKGMQEF LLRASPSKAG PPGTSL |
| 分子量 | 91.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GTP酶SLIP-GC(C8orf80)蛋白的参考文献示例(注：部分文献信息为模拟示例，可能非真实存在)：
1. **文献名称**：**"SLIP-GC, a novel ciliary GTPase, regulates ciliogenesis through microtubule dynamics"**
**作者**：Smith A, et al.
**摘要**：该研究鉴定了C8orf80(SLIP-GC)作为纤毛形成的关键GTP酶，通过重组蛋白实验证明其通过水解GTP调控微管组装，影响纤毛结构和功能。
2. **文献名称**：**"Structural and functional analysis of the human SLIP-GC GTPase domain"**
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析SLIP-GC的GTP结合域结构，揭示其与经典GTP酶的差异，重组蛋白实验显示其活性依赖于Mg²⁺浓度，并提出其可能参与细胞周期调控。
3. **文献名称**：**"C8orf80 deficiency in a mouse model leads to hydrocephalus via disrupted vesicular trafficking"**
**作者**：Wang Y, et al.
**摘要**：利用重组SLIP-GC蛋白验证其在小鼠脑组织中的表达，基因敲除导致脑室扩张，机制可能与GTP酶介导的囊泡运输缺陷相关。
4. **文献名称**：**"Genome-wide association study links SLIP-GC (C8orf80) to neurodegenerative disorders"**
**作者**：Johnson R, et al.
**摘要**：通过GWAS分析发现C8orf80基因变异与阿尔茨海默病风险相关，体外重组蛋白实验表明其GTP酶活性异常可能导致神经元轴突运输障碍。
(注：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索确认。)
The recombinant human GTPase SLIP-GC (C8orf80) is a relatively understudied protein encoded by the C8orf80 gene located on chromosome 8. Structurally, it contains a conserved GTPase domain, characteristic of proteins involved in nucleotide binding and hydrolysis, and a unique C-terminal coiled-coil motif, suggesting roles in protein-protein interactions or oligomerization. SLIP-GC is implicated in spermatogenesis, with studies linking its expression to germ cell differentiation and sperm cell formation, particularly during the transition from round to elongating spermatids. Its GTPase activity may regulate vesicular trafficking or cytoskeletal dynamics critical for sperm maturation. Despite its name, SLIP-GC does not belong to classical GTPase subfamilies (e.g., Ras, Rab, or Rho), indicating specialized functions distinct from well-characterized GTPases. Recombinant SLIP-GC is typically expressed in heterologous systems (e.g., E. coli or mammalian cells) for biochemical studies, enabling analysis of enzymatic properties and interactomes. Limited data exist on its physiological regulators (e.g., GTPase-activating proteins) or pathological relevance, though dysregulation may contribute to male infertility. Further research is needed to elucidate its molecular mechanisms and therapeutic potential.
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