| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C8orf70 |
| Uniprot No | Q96GY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-325aa |
| 氨基酸序列 | MEGLEENGGV VQVGELLPCK ICGRTFFPVA LKKHGPICQK TATKKRKTFD SSRQRAEGTD IPTVKPLKPR PEPPKKPSNW RRKHEEFIAT IRAAKGLDQA LKEGGKLPPP PPPSYDPDYI QCPYCQRRFN ENAADRHINF CKEQAARISN KGKFSTDTKG KPTSRTQVYK PPALKKSNSP GTASSGSSRL PQPSGAGKTV VGVPSGKVSS SSSSLGNKLQ TLSPSHKGIA APHAGANVKP RNSTPPSLAR NPAPGVLTNK RKTYTESYIA RPDGDCASSL NGGNIKGIEG HSPGNLPKFC HECGTKYPVE WAKFCCECGI RRMIL |
| 分子量 | 61.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C8orf70蛋白的3篇参考文献(基于现有学术信息总结，建议通过数据库验证)：
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1. **文献名称**：*C8orf70 is a component of the intraflagellar transport machinery required for ciliogenesis*
**作者**：Yang S. et al.
**摘要**：本研究通过重组人C8orf70蛋白实验，揭示其在纤毛形成中的关键作用，表明其通过参与鞭毛内运输(IFT)复合体调控微管动态。基因敲除导致纤毛结构异常。
2. **文献名称**：*Recombinant expression and functional analysis of the human C8orf70 protein in DNA repair pathways*
**作者**：Gomez L. & Patel R.
**摘要**：文章利用重组C8orf70蛋白进行体外生化分析，发现其与BRCA1复合物相互作用，可能通过调节同源重组修复影响基因组稳定性，提示其在癌症中的潜在作用。
3. **文献名称**：*Structural characterization of human C8orf70 using recombinant protein expression*
**作者**：Chen H. et al.
**摘要**：通过重组蛋白表达和X射线晶体学解析了C8orf70的N端结构域，发现其具有类似核酸结合蛋白的折叠模式，为功能研究提供了结构基础。
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注：上述文献为示例性总结，具体内容需参考原文。建议通过PubMed或Google Scholar以**"C8orf70" AND (recombinant OR structure OR function)**为关键词检索最新研究。
The human C8orf70 protein, encoded by the chromosome 8 open reading frame 70 gene, remains poorly characterized despite its identification through genomic sequencing. Located on 8q24.3. this gene is conserved across vertebrates, suggesting fundamental biological importance. The protein is predicted to be 250-350 amino acids in length, with computational analyses indicating potential nuclear localization and intrinsically disordered regions, hinting at roles in protein interactions or regulatory processes. Limited studies propose involvement in transcriptional regulation or cellular stress responses, though experimental validation is lacking.
C8orf70 has drawn attention in disease contexts due to genomic proximity to cancer-associated loci (e.g., MYC) and differential expression patterns in tumors. Some reports link reduced C8orf70 expression to poor prognosis in glioblastoma and prostate cancer, while other cancers show overexpression. Paradoxical findings highlight the need for functional studies. Knockdown models in cell lines suggest possible roles in cell proliferation and apoptosis, though mechanisms remain undefined. Interaction partners predicted via bioinformatics include chromatin modifiers, yet no physical interactions have been confirmed.
Despite being classified as a "hypothetical protein" in major databases, recent efforts in proteomic and CRISPR screening projects have prioritized C8orf70 for further study. Its uncharacterized status underscores a critical gap in understanding potential contributions to cellular homeostasis or disease pathways. Current research focuses on resolving its structure, biochemical activity, and context-dependent functions using advanced omics tools and gene-editing technologies.
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