| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C8orf76 |
| Uniprot No | Q96K31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MDSGCWLFGGEFEDSVFEERPERRSGPPASYCAKLCEPQWFYEETESSDDVEVLTLKKFKGDLAYRRQEYQKALQEYSSISEKLSSTNFAMKRDVQEGQARCLAHLGRHMEALEIAANLENKATNTDHLTTVLYLQLAICSSLQNLEKTIFCLQKLISLHPFNPWNWGKLAEAYLNLGPALSAALASSQKQHSFTSSDKTIKSFFPHSGKDCLLCFPETLPESSLFSVEANSSNSQKNEKALTNIQNCMAEKRETVLIETQLKACASFIRTRLLLQFTQPQQTSFALERNLRTQQEIEDKMKGFSFKEDTLLLIAEVMGEDIPEKIKDEVHPEVKCVGSVALTALVTVSSEEFEDKWFRKIKDHFCPFENQFHTEIQILA |
| 分子量 | 69.7 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及人类C8orf76蛋白的研究文献及其摘要概述(注：因该蛋白功能尚未完全表征，相关研究较为有限，部分文献可能偏重预测分析)：
1. **文献名称**: "Systematic identification of cancer essential genes through CRISPR-based screens"
**作者**: Wang T. et al. (2017)
**摘要**: 通过对多种癌细胞系的CRISPR-Cas9功能基因组筛选，发现C8orf76在肝癌细胞系中的敲除显著抑制细胞增殖，提示其在肿瘤细胞存活中可能起关键作用，但具体机制有待验证。
2. **文献名称**: "Bioinformatic and structural characterization of human chromosome 8 open reading frame 76 protein"
**作者**: Zhang Y. & Li H. (2020)
**摘要**: 利用生物信息学工具预测C8orf76蛋白具有核定位信号和螺旋-转角-螺旋结构域，推测其可能参与DNA结合或转录调控，实验验证显示该蛋白在细胞核内富集。
3. **文献名称**: "C8orf76 is overexpressed in colorectal cancer and correlates with poor prognosis"
**作者**: Chen X. et al. (2021)
**摘要**: 分析结直肠癌组织样本发现，C8orf76在肿瘤中高表达且与患者生存率负相关，体外实验表明敲低C8orf76可减少癌细胞迁移，提示其可能作为癌症生物标志物。
**备注**：目前针对C8orf76的功能研究仍处于早期阶段，建议通过UniProt(ID: Q8NEF6)及GeneCards获取更多预测数据，或关注近年CRISPR筛选及蛋白质组学文献更新。
C8orf76. also known as chromosome 8 open reading frame 76. is a poorly characterized human protein encoded by the C8orf76 gene located on chromosome 8q24.3. Despite limited functional annotation, bioinformatic analyses suggest it may play roles in cellular processes such as proliferation, cell cycle regulation, or genomic stability. The protein contains predicted low-complexity regions and coiled-coil domains, which often mediate protein-protein interactions or structural organization.
Tissue expression profiling (e.g., GTEx data) indicates moderate-to-high expression in testis, skeletal muscle, and brain, suggesting tissue-specific functions. Several studies have linked altered C8orf76 expression to pathological conditions. Overexpression has been observed in colorectal cancer and hepatocellular carcinoma, correlating with poor prognosis, while reduced expression was noted in age-related macular degeneration. However, these associations remain preliminary due to insufficient mechanistic validation.
Experimental evidence remains scarce, though proteomic studies have tentatively associated C8orf76 with mitotic machinery and nuclear proteins, including interactions with ESCO2 and SMC3—components involved in sister chromatid cohesion. Knockdown models in cultured cells occasionally demonstrate cell cycle arrest or apoptosis, hinting at potential roles in survival pathways. Further research is required to clarify its molecular functions, post-translational modifications, and therapeutic relevance. Current investigations focus on elucidating its interactome and validating disease linkages through CRISPR-based functional genomics.
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