| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C5orf41 |
| Uniprot No | Q8IUR6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MPQPSVSGMDPPFGDAFRSHTFSEQTLMSTDLLANSSDPDFMYELDREMNYQQNPRDNFLSLEDCKDIENLESFTDVLDNEGALTSNWEQWDTYCEDLTKYTKLTSCDIWGTKEVDYLGLDDFSSPYQDEEVISKTPTLAQLNSEDSQSVSDSLYYPDSLFSVKQNPLPSSFPGKKITSRAAAPVCSSKTLQAEVPLSDCVQKASKPTSSTQIMVKTNMYHNEKVNFHVECKDYVKKAKVKINPVQQSRPLLSQIHTDAAKENTCYCGAVAKRQEKKGMEPLQGHATPALPFKETQELLLSPLPQEGPGSLAAGESSSLSASTSVSDSSQKKEEHNYSLFVSDNLGEQPTKCSPEEDEEDEEDVDDEDHDEGFGSEHELSENEEEEEEEEDYEDDKDDDISDTFSEPGYENDSVEDLKEVTSISSRKRGKRRYFWEYSEQLTPSQQERMLRPSEWNRDTLPSNMYQKNGLHHGKYAVKKSRRADVEDLTPNPKKLLQIGNELRKLNKVISDLTPVSELPLTARPRSRKEKNKLASRACRLKKKAQYEANKVKLWGLNTEYDNLLFVINSIKQEIVNRVQNPRDERGPNMGQKLEILIKDTLGLPVAGQTSEFVNQVLEKTAEGNPTGGLVGLRIPTSKV |
| 分子量 | 98.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设的参考文献示例（实际文献可能需要根据最新研究进行验证）：
1. **标题**：*Structural characterization and recombinant expression of human C5orf41 protein*
**作者**：Smith A, et al.
**摘要**：报道了人C5orf41蛋白的重组表达和纯化，通过X射线晶体学解析其结构，并预测其可能参与核苷酸代谢相关酶活性。
2. **标题**：*C5orf41 deficiency disrupts mitochondrial function in mammalian cells*
**作者**：Zhang Y, et al.
**摘要**：利用CRISPR技术敲除C5orf41基因，发现线粒体膜电位受损及ATP合成减少，重组蛋白回补实验证实其在能量代谢中的关键作用。
3. **标题**：*C5orf41 interacts with the mTOR signaling pathway: implications for cancer biology*
**作者**：Johnson R, et al.
**摘要**：通过免疫共沉淀和重组蛋白技术，揭示C5orf41与mTOR通路的相互作用，提示其在肿瘤细胞增殖中的潜在调控功能。
4. **标题**：*Bioinformatic and evolutionary analysis of C5orf41 across species*
**作者**：Brown K, et al.
**摘要**：系统分析了C5orf41的进化保守性及结构域特征，推测其可能为新型水解酶，并设计重组蛋白进行活性验证。
**注**：C5orf41的研究目前可能较少，上述内容为假设性示例，建议通过PubMed或UniProt数据库检索实际文献。
The human C5orf41 protein, encoded by the chromosome 5 open reading frame 41 gene, remains poorly characterized in current scientific literature, reflecting its status as an understudied protein. It is classified as a putative protein-coding gene, with limited functional annotations available in most biological databases. Bioinformatic analyses suggest that C5orf41 may contain conserved structural domains, though their specific roles remain undefined. Tissue expression profiling indicates low-to-moderate mRNA levels across various human tissues, with slightly elevated expression in reproductive and endocrine systems.
Experimental evidence from proteomic studies has detected its presence in cellular compartments, though subcellular localization data remain contradictory. Preliminary studies propose potential involvement in metabolic pathways or cellular signaling processes, with hypothesized connections to nucleotide metabolism based on weak homology to bacterial enzymes. However, these associations lack direct experimental validation.
Notably, genome-wide association studies have implicated the C5orf41 locus in several pathological conditions, including cancer susceptibility and metabolic disorders, though the protein's exact role in these diseases remains speculative. Current challenges in its characterization stem from the absence of specific antibodies, appropriate cellular models, and clear phenotypic associations in knockout studies. Recent advances in CRISPR screening and protein interaction mapping may accelerate functional discovery, but as of now, C5orf41 persists as an enigmatic component of the human proteome warranting further investigation.
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