| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C5orf35 |
| Uniprot No | Q8NE22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MPGRLLRGLW QRWRRYKYRF VPWIALNLSH NPRTLRYVPE ESKDKVISDE DVLGTLLKVF QALFLNDFNK QSEILSMLPE SVKSKYQDLL AVEHQGVKLL ENRHQQQSTF KPEEILYKTL GFSVAQATSS LISAGKGVFV TKGLVPKGAV VSMYPGTVYQ KYEPIFFQSI GNPFIFRCLD GVLIDGNDKG ISKVVYRSCN GRDRLGPLKM SDSTWLTSEI HNPLAVGQYV NNCSNDRAAN VCYQEFDVPA VFPIELKQYL PNIAYSYDKQ SPLRCVVLVA LRDINQGEEL FSNYYTIVS |
| 分子量 | 60.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于**重组人未表征蛋白C5orf35**的3篇代表性文献摘要示例（实际研究中该蛋白可能被更名或研究较少，以下内容基于假设性文献模型整理）：
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1. **《生物信息学分析揭示C5orf35的潜在功能结构》**
*作者：Li X et al. (2020)*
**摘要**：通过生物信息学工具预测C5orf35的跨膜结构域和可能的酶活性位点，提示其可能参与细胞内信号转导或代谢调节，并建议通过重组蛋白表达验证其功能。
2. **《人C5orf35基因在不同癌症组织中的差异表达研究》**
*作者：Wang Y et al. (2018)*
**摘要**：利用RNA测序分析发现C5orf35在乳腺癌和结直肠癌中表达显著上调，可能作为癌症标志物或潜在治疗靶点，并成功在大肠杆菌中重组表达该蛋白用于抗体开发。
3. **《染色体5q31区域未表征蛋白C5orf35的基因定位与进化保守性》**
*作者：Smith J, Johnson R (2016)*
**摘要**：分析了C5orf35在灵长类动物中的进化保守性，发现其启动子区存在高度保守的调控元件，推测其在细胞增殖或分化中发挥作用，并尝试通过HEK293细胞重组表达研究亚细胞定位。
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**说明**：
由于C5orf35可能尚未被充分研究，实际文献可能较少或聚焦于基因定位、表达谱分析或间接功能预测。建议通过NCBI PubMed或UniProt数据库以最新关键词（如新命名、相关通路）检索更新进展。如需具体文献，可提供更详细背景信息进一步筛选。
**Background of Recombinant Human Uncharacterized Protein C5orf35**
The human uncharacterized protein C5orf35, encoded by the *C5orf35* gene on chromosome 5, remains poorly understood due to limited experimental data. Current annotations in databases like UniProt and NCBI describe it as a putative protein with no confirmed functional domains, structural motifs, or established biological roles. Its gene locus lies in a region associated with certain cancers and developmental disorders, though direct links remain speculative.
Recombinant C5orf35 refers to the protein expressed *in vitro* using heterologous systems (e.g., *E. coli* or mammalian cells) for structural or functional studies. Efforts to produce it recombinantly aim to elucidate its biochemical properties, interactions, or post-translational modifications. Preliminary bioinformatics analyses suggest weak homology to proteins involved in nucleic acid binding or chromatin remodeling, but experimental validation is lacking. Tissue expression profiles indicate low-to-moderate mRNA levels in several organs, hinting at potential tissue-specific roles.
Research on C5orf35 is challenged by the absence of specific antibodies, reliable assays, or phenotypic data from knockout models. Despite its obscurity, interest persists due to its genomic context near disease-associated loci and evolutionary conservation across vertebrates, implying functional significance. Further studies leveraging recombinant C5orf35 could clarify its role in cellular pathways or disease mechanisms, bridging critical gaps in human proteome annotation.
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