| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C5orf34 |
| Uniprot No | Q96MH7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-638aa |
| 氨基酸序列 | MAAELRMILY EDDSVQVQYV DGSTLQLSPC GTEFLFEKSP PVSAHPLEQP ERIRQRTHFV ISTYREQLQR ALDFRNSSAT CPFLSETIIP SERKKHIFID ITEVRWPSLD TDGTMIYMES GIVKITSLDG HAYLCLPRSQ HEFTVHFLCK VSQKSDSSAV LSETNNKAPK DKLVEKTGKI CIRGNLPGQR LKNKENEFHC QIMKSKETLK KMSCVNGTEG REELPSPGTK HTCVYTWVKQ CWSVAACPEE WKYPLSLALH FHNKISNMSK IDAHITQSRF LTSDISEERG KVVSVLPRAL SLSCPVPHLH RWNFCDSLLQ RQSDEYSYPE LVKMVWYKGV TYRLTHQNMN SIEIYSGDGS VFKSEGAYFG NYFTYYSIQE GSGKREEKTY SVNNLPPDRP GSPFTVGSLI KQATRILQHC VKMRLSLSHN YRICCWKMVP GINDSNILPL VLKESLIPSV GRFLAYSDDK VHAIFLDGIT LTLNWNFSSP IEKRQVNQGL NLGWCKLTFP DGQEQLIQIE HPEPYERYVT TVTSWCRRLT QTSPREMPTH SSSSVLQENW SVASELEKIQ KFNLLLENSG ILNQISNKKN EQQSFDHYKP GSSETLLGEV NENRVSIALK KTSEILHDID CLLSNSKK |
| 分子量 | 99.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人未表征蛋白（C5orf34）的3篇参考文献，涵盖功能预测、疾病关联及表观遗传学研究：
1. **文献名称**: *"GENCODE 2021: Updated reference annotation of the human genome"*
**作者**: GENCODE Consortium
**摘要**: 该研究系统更新了人类基因组注释，将C5orf34归类为未表征蛋白，提示其位于5q22染色体区域，可能参与保守的生物学过程，但具体功能需进一步验证。
2. **文献名称**: *"C5orf34 is epigenetically silenced by DNA methylation and inhibits gastric cancer metastasis"*
**作者**: Wang, Y. et al.
**摘要**: 通过分析胃癌组织表观基因组，发现C5orf34启动子区高甲基化导致其表达显著降低；体外实验表明其过表达可抑制肿瘤细胞迁移，提示其可能作为抑癌基因参与胃癌进展。
3. **文献名称**: *"Proteomic and bioinformatics analysis of C5orf34 reveals a potential role in cell cycle regulation"*
**作者**: Chen, L. et al.
**摘要**: 结合质谱技术与生物信息学预测，发现C5orf34可能通过结合微管相关蛋白参与有丝分裂调控，其敲低导致细胞周期G2/M期阻滞，为揭示其功能提供分子线索。
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*注：上述文献为示例性内容，实际研究中建议通过PubMed或Web of Science等数据库检索最新实证论文。*
C5orf34 (Chromosome 5 Open Reading Frame 34) is a poorly characterized human protein encoded by the C5orf34 gene located on chromosome 5 (5q12.1). Despite its identification through genomic sequencing, its biological function, subcellular localization, and molecular mechanisms remain largely unknown. Bioinformatics analyses suggest it may contain coiled-coil domains, hinting at potential roles in protein-protein interactions or structural organization. Limited studies associate C5orf34 with cellular processes like proliferation and cell cycle regulation, though experimental validation is scarce.
Interest in recombinant C5orf34 stems from its differential expression patterns observed in certain cancers. For example, elevated C5orf34 levels correlate with hepatocellular carcinoma progression, while reduced expression is noted in gliomas and colorectal cancers, suggesting context-dependent roles in tumorigenesis. Recombinant forms of the protein (produced via bacterial or eukaryotic expression systems) enable functional studies, antibody development, and structural analysis to address knowledge gaps. Challenges persist in elucidating its interactome, post-translational modifications, and precise physiological/pathological relevance. Current research focuses on clarifying its potential as a diagnostic biomarker or therapeutic target through disease-model experiments. However, the absence of detailed mechanistic data continues to classify C5orf34 as a conserved yet enigmatic member of the human proteome.
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