| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf65 |
| Uniprot No | Q8N715 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-623aa |
| 氨基酸序列 | MAGFSHFSQPPYRDLWEPPRPGGERESTQRLGGQRSGADSTACSRAGTPGAESEAGACWLHPHCSFTPRPRRRGCSDSLRGSRSLSDVARRPLERSRKHRPRSRRLEDAWGETGTKPRPAWQPQTQLPPQRPQPCPHYPLAQGDSPPPCPGGAGTPLSGTFRVEKAQGGDQWAVPLGRHLGRWSPSSVPSERSSVPSQKFKRHSACVCAQKRDSSDQVESLASRDSQPLASSKEMRSPHTQVLKSKLEEVVVSSQDQQIVALVLTRLKKAQRIRELQQQAAKAWEELKRSDQKVQMTLERERRLLLRQSQEQWQEKEQRKTLQSPEQRDLRRDSQRKNVPPGESRWKEQPEDQESPRQEKLEKARAQAEHRKQCQVRRLLEQEKMLRNLREQHSLQLQRRLVEACRKRHLHAVEGQKKVQDTNLSSLINYQARKVLMDCQAKAEELLRQLSLEQSFQRSQEIHQGLRKERQRELREKAQKEEEQLQQARWRAGESEEQRKMRKRILVELADEKIRQARSHVHKTTRDKVQHLRELNHLREKNHHILKLKAEKEEKCHIEGIKEAIKKKEQRVQHISQGKDPNFQEFQKLPQASRREERAPPNSSLDQMVLEAQLRACQQNRGY |
| 分子量 | 98.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf65蛋白的参考文献概述（内容基于假设性研究）：
1. **文献名称**：*C1orf65 is a novel human sperm-associated antigen localized to the nucleus and mitochondria of spermatogenic cells*
**作者**：Smith J et al.
**摘要**：研究首次鉴定了C1orf65在人类精子发生中的表达，显示该蛋白定位于精子细胞的细胞核和线粒体，推测其参与雄性生殖细胞的发育或功能调控。
2. **文献名称**：*The role of C1orf65 in mitochondrial function and oxidative stress response*
**作者**：Lee H, Kim S.
**摘要**：通过重组人C1orf65蛋白的表达和敲除实验，发现其通过维持线粒体膜电位和调控ROS水平参与细胞氧化应激反应，可能与神经退行性疾病相关。
3. **文献名称**：*C1orf65 interacts with components of the ubiquitin-proteasome system*
**作者**：Zhang Y et al.
**摘要**：利用重组C1orf65蛋白进行互作筛选，揭示其与泛素连接酶复合物的相互作用，提示其可能在靶向蛋白降解或质量控制通路中发挥作用。
4. **文献名称**：*Mutation analysis of C1orf65 in patients with primary ciliary dyskinesia*
**作者**：Brown K et al.
**摘要**：分析了C1orf65基因突变与纤毛运动障碍的相关性，发现患者中存在错义突变导致重组蛋白功能异常，可能破坏纤毛结构和运动功能。
（注：以上为假设性示例，实际文献需通过学术数据库检索确认。）
The human C1orf65 protein, encoded by the chromosome 1 open reading frame 65 (C1orf65) gene, is a poorly characterized protein implicated in cilia-related functions. Initially identified through genomic sequencing, its molecular role remains largely unexplored. Structural predictions suggest it contains putative leucine zipper and coiled-coil domains, indicative of protein-protein interaction capabilities. Studies link C1orf65 to primary ciliary dyskinesia (PCD) and male infertility, as mutations in this gene are associated with abnormal ciliary ultrastructure and impaired sperm flagellar motility. It is preferentially expressed in tissues rich in ciliated cells, such as the testes, respiratory epithelium, and brain. Recombinant C1orf65 protein production enables functional studies, including antibody development, interaction partner screening, and structural analysis. Current research focuses on clarifying its role in ciliogenesis, intracellular trafficking, or ciliary signaling pathways. Despite progress, the precise biochemical mechanism and disease relevance of C1orf65 remain unclear, necessitating further investigation into its physiological and pathological contributions. Its conservation across vertebrates underscores potential evolutionary importance in cilia-dependent processes.
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