| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf59 |
| Uniprot No | Q5T8I9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-393aa |
| 氨基酸序列 | MEENNLQCSS VVDGNFEEVP RETAIQFKPP LYRQRYQFVK NLVDQHEPKK VADLGCGDTS LLRLLKVNPC IELLVGVDIN EDKLRWRGDS LAPFLGDFLK PRDLNLTITL YHGSVVERDS RLLGFDLITC IELIEHLDSG DLARFPEVVF GYLSPSMIVI STPNSEFNPL FPSVTLRDSD HKFEWTRMEF QTWALYVANR YDYSVEFTGV GEPPAGAENV GYCTQIGIFR KNGGKATESC LSEQHDQHVY KAVFTTSYPS LQQERFFKLV LVNEVSQQVE SLRVSHLPRR KEQAGERGDK PKDIGGSKAP VPCFGPVFTE VEKAKIENSP TPFCVGDKFF VPLQRLLAYP KLNRLCANEE MMRSVIADSI PLSSDGSAVV ADLRNYFDEQ FEF |
| 分子量 | 44.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf59蛋白的参考文献示例（基于研究主题和领域常见方向的假设性示例，具体文献需通过学术数据库进一步验证）：
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1. **文献名称**: **"C1orf59 is required for ribosomal RNA biogenesis and cell proliferation"**
**作者**: Zhou et al., 2018
**摘要**: 通过重组C1orf59蛋白的体外实验，研究发现其与核糖体组装因子URB1相互作用，共同调控核糖体RNA前体的剪切，并影响细胞周期进程。基因敲低导致细胞增殖抑制和核仁结构异常。
2. **文献名称**: **"Recombinant C1orf59 regulates TGF-β signaling in hepatocellular carcinoma metastasis"**
**作者**: Li et al., 2020
**摘要**: 研究在肝癌细胞中使用重组C1orf59蛋白，发现其通过增强TGF-β通路活性促进肿瘤细胞迁移和侵袭。Western blot分析显示其调控SMAD蛋白磷酸化。
3. **文献名称**: **"Biochemical characterization of C1orf59 in pre-rRNA processing"**
**作者**: Smith et al., 2017
**摘要**: 通过大肠杆菌表达系统纯化重组C1orf59蛋白，结合免疫共沉淀实验，证实其参与18S rRNA成熟过程，并与核糖体生物发生复合物（例如UTP复合物）存在相互作用。
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**注意**：以上文献为假设性示例，C1orf59的研究可能有限，建议通过PubMed或Google Scholar以关键词“C1orf59”、“recombinant C1orf59”或“KIAA1530”检索最新文献。真实研究中，C1orf59可能被关联于核糖体RNA加工、癌症或纤毛功能等领域。
C1orf59 (Chromosome 1 Open Reading Frame 59), also known as FAM134C or RANBP1, is a human protein encoded by the C1orf59 gene located on chromosome 1p34.3. Structurally, it contains a conserved FAM134 domain, which is characteristic of the FAM134 protein family involved in membrane dynamics and autophagy. The protein is characterized by coiled-coil regions and potential transmembrane domains, suggesting roles in intracellular trafficking or organelle membrane organization.
Although its precise biological functions remain understudied, C1orf59 has been implicated in Golgi-ER retrograde transport and vesicle formation. Emerging evidence links it to neurological and metabolic processes. For instance, altered C1orf59 expression has been observed in neurodegenerative disorders and certain cancers. In colorectal cancer, it was reported as a tumor suppressor candidate, with downregulation correlating with poor prognosis.
Recombinant C1orf59 protein is typically produced using bacterial or mammalian expression systems for functional studies. It serves as a tool to investigate protein-protein interactions, signaling pathways, and cellular localization. Antibodies targeting recombinant C1orf59 are utilized in Western blotting, immunofluorescence, and immunohistochemistry to explore its tissue distribution and disease associations. Further research is needed to fully elucidate its molecular mechanisms and therapeutic potential in human pathologies.
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