| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C15orf15 |
| Uniprot No | Q9UHA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-150aa |
| 氨基酸序列 | MRIEKCYFCSGPIYPGHGMMFVRNDCKVFRFCKSKCHKNFKKKRNPRKVRWTKAFRKAAGKELTVDNSFEFEKRRNEPIKYQRELWNKTIDAMKRVEEIKQKRQAKFIMNRLKKNKELQKVQDIKEVKQNIHLIRAPLAGKGKQLEEKMV |
| 分子量 | 42.24 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C15orf15蛋白的参考文献摘要，基于现有研究趋势推测可能的研究方向(*注：C15orf15研究尚有限，以下为模拟示例*)：
1. **文献名称**：*Expression and purification of recombinant human C15orf15 protein in Escherichia coli*
**作者**：Zhang L, et al.
**摘要**：本研究建立了C15orf15蛋白在大肠杆菌中的重组表达系统，优化了诱导条件并通过镍柱亲和层析纯化。Western blot验证了蛋白的高效表达，为进一步功能研究奠定基础。
2. **文献名称**：*C15orf15 interacts with mitochondrial ribosomal proteins and supports oxidative phosphorylation*
**作者**：Kim S, Park JH.
**摘要**：通过免疫共沉淀结合质谱分析，发现重组C15orf15蛋白与线粒体核糖体亚基相互作用，敲低实验显示其缺失导致ATP合成下降，提示其在线粒体能量代谢中的潜在作用。
3. **文献名称**：*Structural characterization of human C15orf15 using NMR spectroscopy*
**作者**：Garcia-Ruiz E, et al.
**摘要**：首次报道重组C15orf15蛋白的核磁共振结构解析，发现其含有一个保守的α-螺旋结构域，可能参与蛋白-核酸相互作用。
4. **文献名称**：*C15orf15 promotes cancer cell proliferation via MAPK pathway activation*
**作者**：Wang Y, et al.
**摘要**：在胃癌细胞中过表达重组C15orf15蛋白，结果显示其通过激活ERK信号通路促进细胞增殖，提示其作为肿瘤治疗靶点的可能性。
**注意**：以上文献为假设性示例，实际研究可能有限。建议通过**PubMed**或**Google Scholar**以“C15orf15 recombinant protein”等关键词检索最新文献。
The human C15orf15 protein, encoded by the *C15orf15* gene (chromosome 15 open reading frame 15), is a relatively understudied protein with emerging roles in cellular homeostasis and disease. Located on chromosome 15q22.31. this gene is also termed *FAM213A* (family with sequence similarity 213 member A) and is evolutionarily conserved across mammals. The protein is characterized by a thioredoxin-like fold, suggesting potential redox-regulatory functions, though its precise enzymatic activity remains unclear. Studies link C15orf15 to antioxidant defense systems, particularly in mitigating oxidative stress by interacting with peroxiredoxins or modulating reactive oxygen species (ROS) levels. It is broadly expressed in tissues, including bone marrow, thyroid, and liver, with elevated levels observed in certain cancers, such as hepatocellular carcinoma. In bone biology, C15orf15 is implicated in osteoblast differentiation and bone mineralization, with mutations associated with rare skeletal disorders like osteogenesis imperfecta. Recent research highlights its role in mitochondrial function and apoptosis regulation, potentially influencing metabolic and degenerative diseases. Despite progress, its exact molecular mechanisms and physiological significance require further elucidation. Recombinant C15orf15 protein is utilized in functional studies to explore its interactions and therapeutic potential, particularly in oxidative stress-related pathologies.
×
