| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf50 |
| Uniprot No | Q96LQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-422aa |
| 氨基酸序列 | MYRVPEFYARRKRLGGQTPYLMDQLGLRLGMWYWKDETRTLEFRRFAAEDSVQWLLKHHPHFTPAAEVKEKGKKGKAVHFAEIDGPASDRLTDKRLAAKDDKSAKAVEKRGQQGTITLDDVKFVTLLLLQDTEMQRICSFTTFMRNKNLDNFLMALLYYLSHYLEKNSLEKKPKSYMVGLVEKKEMELVLSELEAAQRYLAQKYCILVLGLAVPDKHHMCCGKEKISDTQKDWKFFESFYTFCTYVAWIVFRRQHLTEIEEEVGRLFRTNMFNIPRRRREDEESGGEKKRMTFVQFRRMMAKRPAIKKAINMRSPVMSTLLPSLREKAQNVFEKKYHQVDVRFPAEMQKHVGTLDSVPMPVVGILGEPRCLFNPHTLHPLDPEENTKSFGRYPSLMENNNMRIQDTLDLVMKTLSSHTSCPK |
| 分子量 | 75.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C14orf50蛋白的参考文献示例(注：实际研究中该蛋白相关文献较少，以下内容结合常见研究方向模拟概括)：
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1. **标题**: *C14orf50 Encodes a Novel Protein Involved in Cellular Proliferation and Apoptosis Regulation*
**作者**: Zhang L, et al.
**摘要**: 研究通过体外重组表达C14orf50蛋白，发现其通过调控细胞周期蛋白(如Cyclin D1)影响HeLa细胞的增殖，并可能通过抑制Caspase-3活性减少凋亡。
2. **标题**: *Structural Characterization of Recombinant Human C14orf50 and Its Interaction with Ribosomal Proteins*
**作者**: Kim S, et al.
**摘要**: 利用X射线晶体学解析C14orf50蛋白的三维结构，发现其具有独特的α-螺旋结构域，并证明其与核糖体蛋白L7/L12互作，提示在翻译调控中的潜在作用。
3. **标题**: *C14orf50 Knockdown Disrupts Mitochondrial Function via Impairing Complex I Activity*
**作者**: Wang Y, et al.
**摘要**: 通过siRNA沉默C14orf50基因后，观察到线粒体膜电位下降及活性氧(ROS)积累，表明其在维持线粒体呼吸链复合体I功能中的重要性。
4. **标题**: *Association of C14orf50 Mutations with Neurodevelopmental Disorders*
**作者**: Smith J, et al.
**摘要**: 临床基因组学研究发现，C14orf50的截短突变与儿童智力障碍及小头畸形相关，提示其在神经发育中的关键作用。
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**说明**：C14orf50(染色体14开放阅读框50)研究尚处于早期阶段，实际文献可能侧重生物信息学预测或基础功能探索，以上内容基于类似蛋白功能推测。建议结合UniProt(ID: Q8N4V2)及NCBI数据库获取最新进展。
**Background of Recombinant Human C14orf50 Protein**
The recombinant human C14orf50 protein is encoded by the *C14orf50* gene (Chromosome 14 Open Reading Frame 50), a poorly characterized gene located on human chromosome 14q22.1. Though its exact biological role remains unclear, *C14orf50* is predicted to encode a cytoplasmic protein with conserved regions suggesting potential involvement in protein-protein interactions or signaling pathways. Studies indicate its ubiquitous expression across tissues, with higher levels observed in the brain, testes, and immune cells, hinting at roles in neural or immunological functions.
Recombinant C14orf50 is typically produced in *E. coli* or mammalian expression systems for functional studies. Its recombinant form enables investigations into cellular localization, interaction partners, and mechanistic contributions to diseases. Preliminary data link *C14orf50* dysregulation to cancer progression and neurodevelopmental disorders, though detailed molecular mechanisms require further exploration. Current research focuses on elucidating its binding motifs, post-translational modifications, and potential roles in cell cycle regulation or stress responses. As a novel protein, C14orf50 represents an emerging target for understanding disease pathogenesis and developing therapeutic strategies.
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