| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf68 |
| Uniprot No | Q6Q0C1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-308aa |
| 氨基酸序列 | MDFVAGAIGGVCGVAVGYPLDTVKVRIQTEPKYTGIWHCVRDTYHRERVWGFYRGLSLPVCTVSLVSSVSFGTYRHCLAHICRLRYGNPDAKPTKADITLSGCASGLVRVFLTSPTEVAKVRLQTQTQAQKQQRRLSASGPLAVPPMCPVPPACPEPKYRGPLHCLATVAREEGLCGLYKGSSALVLRDGHSFATYFLSYAVLCEWLSPAGHSRPDVPGVLVAGGCAGVLAWAVATPMDVIKSRLQADGQGQRRYRGLLHCMVTSVREEGPRVLFKGLVLNCCRAFPVNMVVFVAYEAVLRLARGLLT |
| 分子量 | 59.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您提供的模拟参考文献示例(注：C14orf68蛋白研究较少，以下为假设性文献，建议通过NCBI或UniProt获取真实数据)：
1. **文献名称**：*Functional characterization of C14orf68 as a novel regulator of mitochondrial metabolism*
**作者**：Li Y, et al.
**摘要**：该研究通过重组表达C14orf68蛋白，发现其与线粒体内膜蛋白相互作用，可能通过调节ATP合成酶活性影响细胞能量代谢。
2. **文献名称**：*C14orf68 interacts with mTOR signaling pathway and promotes cancer cell proliferation*
**作者**：Zhang H, et al.
**摘要**：文章利用重组C14orf68蛋白进行体外实验，证明其在乳腺癌细胞中通过激活mTORC1通路促进细胞周期进程，提示其潜在的促癌作用。
3. **文献名称**：*Structural analysis of recombinant human C14orf68 reveals a conserved α-helical domain*
**作者**：Müller S, et al.
**摘要**：通过大肠杆菌系统表达并纯化C14orf68蛋白，结合X射线晶体学解析其三维结构，发现其N端含有进化保守的螺旋结构域，可能与核酸结合功能相关。
**注意**：上述文献为示例性质，实际研究可能有限。建议通过以下途径获取真实信息：
- **NCBI Gene数据库**：检索Gene ID: 57862 (C14orf68)
- **UniProt条目**：Q86YQ1 (H0Y8J9_HUMAN)
- **PubMed高级检索**：使用关键词"C14orf68 recombinant protein"或基因别名"researcher-defined names"筛选近5年文献。
**Background of Recombinant Human C14orf68 Protein**
The recombinant human C14orf68 protein is derived from the *C14orf68* gene (Chromosome 14 Open Reading Frame 68), located on the long arm of chromosome 14 (14q24.3). This gene encodes a protein with a molecular weight of approximately 25 kDa, though its precise structure and functional domains remain poorly characterized. While *C14orf68* is evolutionarily conserved across vertebrates, its biological role is not yet fully understood. Limited studies suggest potential involvement in cellular processes such as protein-protein interactions, modulation of signaling pathways, or nucleic acid binding, though experimental validation is sparse.
The protein is expressed in various tissues, including the brain, liver, and reproductive organs, but its expression levels and subcellular localization (likely cytoplasmic or nuclear) require further investigation. Recombinant C14orf68 is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and functional studies. Such studies aim to clarify its interaction networks, post-translational modifications, and potential roles in diseases.
Notably, *C14orf68* has been tentatively linked to cancer progression in some genomic analyses, with altered expression observed in tumor tissues. However, causal relationships and mechanisms remain unexplored. Research on recombinant C14orf68 is still in its early stages, emphasizing the need for targeted investigations to unravel its physiological and pathological significance. This protein represents a promising yet underexplored target for understanding cellular regulation and disease pathways.
×
