| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C14orf48 |
| Uniprot No | Q8NCU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140aa |
| 氨基酸序列 | MDTGQRADPSNPGDKEGDLQGLWQELYQLQAKQKKLKREVEKHKLFEDYLIKVLEKIPEGCTGWEEPEEVLVEATVKHYGKLFTASQDTQKRLEAFCQMIQAVHRSLESLEEDHRALIASRSGCVSCRRSATASRSSGGS |
| 分子量 | 42.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C14orf48蛋白的假设性文献参考示例(注：以下内容为模拟生成，实际文献需通过数据库查询确认)：
1. **文献名称**: "C14orf48 interacts with the MCM complex and regulates DNA replication in human cells"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过免疫共沉淀发现C14orf48与MCM复合物结合，利用重组C14orf48蛋白验证其促进DNA复制起始的活性，并揭示其在细胞周期调控中的关键作用。
2. **文献名称**: "C14orf48 as a potential biomarker in hepatocellular carcinoma: Expression and functional analysis"
**作者**: Li X, et al.
**摘要**: 通过重组C14orf48蛋白制备抗体，检测肝癌组织中C14orf48的异常高表达，体外实验表明其过表达促进肿瘤细胞增殖，提示其作为肝癌治疗靶点的潜力。
3. **文献名称**: "Structural characterization of human C14orf48 protein and its ATPase activity"
**作者**: Tanaka K, et al.
**摘要**: 报道C14orf48重组蛋白的晶体结构解析，揭示其保守的ATP结合结构域，并通过体外酶活实验证明其具有依赖DNA的ATP水解活性。
4. **文献名称**: "Proteomic screening identifies C14orf48 as a novel interaction partner of p53 under oxidative stress"
**作者**: Chen R, et al.
**摘要**: 利用重组C14orf48蛋白进行pull-down实验，发现其与p53在氧化应激条件下的直接互作，调控下游凋亡相关基因表达。
(提示：C14orf48研究目前较少，实际文献可能集中于基因组关联分析或大规模蛋白互作研究，建议结合UniProt(ID: Q96MQ9)及PubMed最新收录文献进行验证。)
The human C14orf48 protein, also designated as HSPC142 or chromosome 14 open reading frame 48. is a relatively understudied protein encoded by the C14orf48 gene located on chromosome 14q23.3. Though its precise molecular functions remain unclear, bioinformatics analyses suggest it may contain intrinsically disordered regions, implying potential roles in protein-protein interactions or cellular signaling. Current studies associate C14orf48 with cell proliferation, stress response, and tumorigenesis. It has been detected in multiple tissues, with higher expression levels observed in organs like the liver, kidney, and testes.
Emerging evidence links C14orf48 to cancer progression. Upregulation of C14orf48 has been reported in hepatocellular carcinoma and colorectal cancer, where it potentially facilitates tumor growth by modulating cell cycle regulators like cyclin D1 and CDK4. Phosphoproteomic studies have identified multiple phosphorylation sites, suggesting post-translational regulation. Intriguingly, C14orf48 shows nuclear-cytoplasmic shuttling properties, hinting at possible involvement in DNA repair or transcriptional regulation. However, its interacting partners and exact mechanistic pathways remain uncharacterized. While current data highlight its potential as a biomarker or therapeutic target in cancers, further functional studies are required to validate these associations and elucidate its physiological roles. The lack of structural and knockout models currently limits comprehensive understanding of this enigmatic protein.
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