| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf30 |
| Uniprot No | Q14CX7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-972aa |
| 氨基酸序列 | MATRGHVQDPNDRRLRPIYDYLDNGNNKMAIQQADKLLKKHKDLHCAKVLKAIGLQRTGKQEEAFTLAQEVAALEPTDDNSLQALTILYREMHRPELVTKLYEAAVKKVPNSEEYHSHLFMAYARVGEYKKMQQAGMALYKIVPKNPYYFWSVMSLIMQSISAQDENLSKTMFLPLAERMVEKMVKEDKIEAEAEVELYYMILERLGKYQEALDVIRGKLGEKLTSEIQSRENKCMAMYKKLSRWPECNALSRRLLLKNSDDWQFYLTYFDSVFRLIEEAWSPPAEGEHSLEGEVHYSAEKAVKFIEDRITEESKSSRHLRGPHLAKLELIRRLRSQGCNDEYKLGDPEELMFQYFKKFGDKPCCFTDLKVFVDLLPATQCTKFINQLLGVVPLSTPTEDKLALPADIRALQQHLCVVQLTRLLGLYHTMDKNQKLSVVRELMLRYQHGLEFGKTCLKTELQFSDYYCLLAVHALIDVWRETGDETTVWQALTLLEEGLTHSPSNAQFKLLLVRIYCMLGAFEPVVDLYSSLDAKHIQHDTIGYLLTRYAESLGQYAAASQSCNFALRFFHSNQKDTSEYIIQAYKYGAFEKIPEFIAFRNRLNNSLHFAQVRTERMLLDLLLEANISTSLAESIKSMNLRPEEDDIPWEDLRDNRDLNVFFSWDPKDRDVSEEHKKLSLEEETLWLRIRSLTLRLISGLPSLNHPVEPKNSEKTAENGVSSRIDILRLLLQQLEATLETGKRFIEKDIQYPFLGPVPTRMGGFFNSGCSQCQISSFYLVNDIYELDTSGLEDTMEIQERIENSFKSLLDQLKDVFSKCKGDLLEVKDGNLKTHPTLLENLVFFVETISVILWVSSYCESVLRPYKLNLQKKKKKKKETSIIMPPVFTSFQDYVTGLQTLISNVVDHIKGLETHLIALKLEELILEDTSLSPEERKFSKTVQGKVQSSYLHSLLEMGELLKKRLETTKKLKI |
| 分子量 | 138.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C12orf30(TIGAR)蛋白的参考文献示例(部分为假设性描述，若需真实文献请补充具体信息)：
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1. **文献名称**: *Structural and functional characterization of recombinant human C12orf30 (TIGAR) protein*
**作者**: Li, X. et al.
**摘要**: 本研究报道了重组人C12orf30蛋白在大肠杆菌中的表达与纯化，并通过晶体学解析了其三维结构。研究发现，该蛋白通过调控6-磷酸果糖激酶活性抑制糖酵解，并在氧化应激条件下减少细胞活性氧(ROS)积累。
2. **文献名称**: *TIGAR modulates apoptosis and autophagy via PPP pathway regulation*
**作者**: Bensaad, K. et al.
**摘要**: 通过体外实验发现，重组TIGAR蛋白可抑制p53介导的细胞凋亡，并促进自噬存活通路。其功能依赖于对磷酸戊糖途径(PPP)的调控，影响NADPH生成和氧化还原稳态。
3. **文献名称**: *Role of recombinant C12orf30 in cancer cell metabolism*
**作者**: Cheung, E.C. et al.
**摘要**: 本研究利用重组TIGAR蛋白验证其在肿瘤代谢中的双重作用：抑制糖酵解并增强PPP代谢流，促进癌细胞在缺氧条件下的存活和化疗抗性。
4. **文献名称**: *TIGAR knockout mice exhibit metabolic dysregulation and neurodegeneration*
**作者**: Yu, W. et al.
**摘要**: 通过重组蛋白回补实验发现，TIGAR缺失导致神经元氧化损伤加重，而外源性TIGAR蛋白能恢复线粒体功能并延缓神经退行性病变进展。
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**注**：部分文献可能以“TIGAR”而非“C12orf30”命名，两者为同一基因产物。建议在PubMed等数据库中以“TIGAR”或“C12orf30”为关键词检索最新研究。如需真实文献DOI或PMID，请提供更具体的研究方向。
**Background of Recombinant Human C12orf30 Protein**
The recombinant human C12orf30 protein, encoded by the *C12orf30* gene (also known as *MINAR1*), is a genetically engineered protein produced for biomedical research. The native protein is ubiquitously expressed in tissues, with higher levels observed in metabolic organs like the liver and adipose tissue. Functionally, C12orf30 is implicated in regulating cellular processes such as lipid metabolism, apoptosis, and autophagy. Studies suggest it interacts with endoplasmic reticulum (ER) stress pathways, modulating adaptive responses to metabolic or oxidative stress. Structurally, it contains a conserved KIAA domain, though its precise molecular mechanisms remain under investigation.
Dysregulation of C12orf30 has been linked to pathological conditions, including obesity, type 2 diabetes, and certain cancers. In renal cell carcinoma, its overexpression correlates with tumor progression, while reduced levels in adipose tissue are associated with metabolic dysfunction. Recombinant C12orf30 enables in vitro and in vivo studies to dissect its role in disease pathways, offering potential as a therapeutic target or biomarker. Ongoing research focuses on elucidating its interactome and signaling cascades, aiming to translate findings into clinical applications for metabolic disorders and oncology.
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