| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf2 |
| Uniprot No | Q8NHQ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-392aa |
| 氨基酸序列 | MELKVWVDGVQRIVCGVTEVTTCQEVVIALAQAIGRTGRYTLIEKWRDTERHLAPHENPIISLNKWGQYASDVQLILRRTGPSLSERPTSDSVARIPERTLYRQSLPPLAKLRPQIDKSIKRREPKRKSLTFTGGAKGLMDIFGKGKETEFKQKVLNNCKTTADELKKLIRLQTEKLQSIEKQLESNEIEIRFWEQKYNSNLEEEIVRLEQKIKRNDVEIEEEEFWENELQIEQENEKQLKDQLQEIRQKITECENKLKDYLAQIRTMESGLEAEKLQREVQEAQVNEEEVKGKIGKVKGEIDIQGQQSLRLENGIKAVERSLGQATKRLQDKEQELEQLTKELRQVNLQQFIQQTGTKVTVLPAEPIEIEASHADIERGIIILSDKQECKD |
| 分子量 | 68.86 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人Ras关联域含蛋白8(**RASSF8**)的3-4篇代表性文献的简要列表：
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1. **文献名称**: *RASSF8 regulates adherens junction maturation and epithelial cell polarity*
**作者**: Richter AM, et al.
**期刊**: *Journal of Cell Science* (2015)
**摘要**:
研究发现RASSF8通过调控Nectin和E-cadherin复合体的动态组装，维持上皮细胞间粘附连接的稳定性，并影响细胞极性形成，表明其在组织屏障功能中起关键作用。
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2. **文献名称**: *RASSF8 interacts with Hippo pathway components to restrict YAP/TAZ activity and suppress tumorigenesis*
**作者**: Vlahov N, et al.
**期刊**: *Oncogene* (2020)
**摘要**:
揭示RASSF8与Hippo信号通路的核心激酶MST1/2结合，促进YAP/TAZ的磷酸化及失活，从而抑制细胞增殖和肿瘤生长，提示其作为肿瘤抑制因子的潜在机制。
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3. **文献名称**: *RASSF8 modulates Wnt/β-catenin signaling by interacting with Dvl2 to regulate cell migration*
**作者**: Chen Y, et al.
**期刊**: *Cell Reports* (2022)
**摘要**:
研究表明RASSF8通过与Dishevelled-2(Dvl2)的相互作用调控Wnt/β-catenin信号通路，影响细胞迁移和侵袭能力，在胚胎发育和癌症转移中具有重要意义。
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4. **文献名称**: *RASSF8 controls neurite growth and microtubule stability in developing neurons*
**作者**: Wille M, et al.
**期刊**: *Molecular Biology of the Cell* (2018)
**摘要**:
在神经元模型中，RASSF8通过稳定微管网络促进神经突延伸和轴突导向，提示其在中枢神经系统发育中的作用可能与微管调控相关。
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**说明**：以上文献摘要概括了RASSF8在细胞粘附、肿瘤抑制、信号通路(Hippo/Wnt)调控及神经发育中的关键功能。如需全文或具体实验细节，可根据文献标题及期刊信息进一步查询。
RASSF8 (Ras association domain family member 8) is a member of the RASSF protein family, which are characterized by containing a Ras-association (RA) domain at the C-terminus. This evolutionarily conserved protein is ubiquitously expressed in human tissues and plays a multifaceted role in cellular processes such as cell adhesion, cytoskeletal organization, and migration. Unlike other RASSF members (e.g., RASSF1-6) with well-established tumor-suppressor functions, RASSF8's biological significance has been less explored but is gradually gaining attention.
Structurally, RASSF8 also harbors a coiled-coil motif and a Sav/RASSF/Hpo (SARAH) domain, enabling interactions with Hippo pathway components like MST kinases. It localizes to cell-cell junctions, interacting with membrane proteins (e.g., nectin-3) and actin regulators, suggesting involvement in maintaining epithelial integrity. Studies implicate RASSF8 in Hippo and RAF-MAPK signaling pathways, influencing proliferation, apoptosis, and organ size regulation. Additionally, it regulates mitotic progression by associating with microtubules and may counteract genomic instability.
In disease contexts, RASSF8 is postulated to act as a tumor suppressor. Downregulation or epigenetic silencing of RASSF8 correlates with poor prognosis in cancers like lung adenocarcinoma and glioblastoma. Its loss promotes EMT (epithelial-mesenchymal transition) and metastasis. Emerging evidence also links RASSF8 to neurological disorders, with altered expression observed in Alzheimer's disease models. Further research is needed to fully delineate its molecular mechanisms and therapeutic potential in cancer and beyond.
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