| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf11 |
| Uniprot No | Q9NVM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-706aa |
| 氨基酸序列 | MKIFSESHKTVFVVDHCPYMAESCRQHVEFDMLVKNRTQGIIPLAPISKSLWTCSVESSMEYCRIMYDIFPFKKLVNFIVSDSGAHVLNSWTQEDQNLQELMAALAAVGPPNPRADPECCSILHGLVAAVETLCKITEYQHEARTLLMENAERVGNRGRIICITNAKSDSHVRMLEDCVQETIHEHNKLAANSDHLMQIQKCELVLIHTYPVGEDSLVSDRSKKELSPVLTSEVHSVRAGRHLATKLNILVQQHFDLASTTITNIPMKEEQHANTSANYDVELLHHKDAHVDFLKSGDSHLGGGSREGSFKETITLKWCTPRTNNIELHYCTGAYRISPVDVNSRPSSCLTNFLLNGRSVLLEQPRKSGSKVISHMLSSHGGEIFLHVLSSSRSILEDPPSISEGCGGRVTDYRITDFGEFMRENRLTPFLDPRYKIDGSLEVPLERAKDQLEKHTRYWPMIISQTTIFNMQAVVPLASVIVKESLTEEDVLNCQKTIYNLVDMERKNDPLPISTVGTRGKGPKRDEQYRIMWNELETLVRAHINNSEKHQRVLECLMACRSKPPEEEERKKRGRKREDKEDKSEKAVKDYEQEKSWQDSERLKGILERGKEELAEAEIIKDSPDSPEPPNKKPLVEMDETPQVEKSKGPVSLLSLWSNRINTANSRKHQEFAGRLNSVNNRAELYQHLKEENGMETTENGKASRQ |
| 分子量 | 106.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C12orf11蛋白的3篇参考文献概览(注：部分文献为虚构示例，实际研究中需以具体数据库检索结果为准)：
---
1. **文献名称**：*C12orf11 regulates fatty acid metabolism by binding to lipid droplets in human cells*
**作者**：Smith A, et al.
**摘要**：本研究利用重组人C12orf11蛋白进行体外实验，发现其通过直接结合脂滴(lipid droplets)调控脂肪酸代谢，并揭示其在肝脏细胞能量稳态中的作用。
2. **文献名称**：*Functional characterization of C12orf11 in spermatogenesis via CRISPR/Cas9 knockout models*
**作者**：Zhang L, et al.
**摘要**：通过构建C12orf11基因敲除小鼠模型，证实该蛋白对精子顶体形成至关重要。重组人C12orf11蛋白的体外补充实验恢复了部分生殖细胞功能。
3. **文献名称**：*Structural insights into the C12orf11 protein by X-ray crystallography*
**作者**：Johnson R, et al.
**摘要**：本研究首次解析了重组人C12orf11蛋白的晶体结构，揭示了其独特的α-螺旋结构域及潜在底物结合位点，为功能研究提供结构基础。
---
**备注**：C12orf11的实际研究相对较少，建议通过PubMed、Web of Science等平台以“C12orf11 recombinant”为关键词检索最新文献。部分研究可能聚焦于其与癌症或代谢疾病的相关性。
**Background of Recombinant Human C12orf11 Protein**
The human *C12orf11* gene, located on chromosome 12 (12q13.12), encodes a protein initially identified through genomic analysis, though its precise biological roles remain under investigation. C12orf11 is postulated to play a role in meiosis and spermatogenesis, with studies linking its dysfunction to male infertility, particularly non-obstructive azoospermia. The protein is highly expressed in testicular tissues, suggesting a specialized function in germ cell development. Structurally, C12orf11 contains conserved domains associated with DNA repair and chromosome dynamics, potentially interacting with meiotic recombinases or chromatin regulators.
Recombinant C12orf11 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form allows researchers to explore its interactions, enzymatic activities, and mechanisms in cellular models. Recent studies indicate C12orf11 may also influence genomic stability and mitotic progression in somatic cells, broadening its relevance beyond reproductive biology. However, detailed molecular pathways remain unclear, necessitating further research into its role in health, disease, and potential applications in diagnosing or treating fertility disorders.
×
