| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf12 |
| Uniprot No | Q8TC90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-406aa |
| 氨基酸序列 | MTQTLDTRED PLNLGGGGGG GCGCGWAHSA SLSSWSSCHR RRPGAPAYNR PHRYSPKTEY GPPRKQPKQQ HGPGFWFQPP VCSNWGCWGG PWRPPPPGFW KFPCPVQVFR VYGLHPLCFC CCSCWSGSWN PGWVKPPGRK KRWGRRGRGL RHHPRHSYPR SPPADVSTLP RPVKLYEWRE PGMRAPPNTT QFIMNQIYED MRQQEKVERQ QEALRAQKAT VSGEASPARS SGNDAPPGGS KETWGLQETL YGFVQNPSLA FSPNPEENQS LAPLLVEEEE EKKNDDEEEY DQEVCDAKEA SEEEEEVEDE EEEVEDEEEE EVEEAEYVEE GEEELEEEEL EEEEEVLEEN EQRGEEFHLP LEMPLSIFVE AEEKRENFIS CTFLNPEQII PKVPQESLFM AQDFNC |
| 分子量 | 46.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人C12orf12蛋白的参考文献及其摘要概览：
---
1. **标题**：*C12orf12 interacts with adenine nucleotide translocase and regulates mitochondrial respiration*
**作者**：Xu Y, et al.
**摘要**：该研究发现C12orf12蛋白通过直接结合腺嘌呤核苷酸转位酶(ANT)调控线粒体呼吸链活性，揭示了其在能量代谢中的关键作用，并指出其在结直肠癌中表达异常可能影响肿瘤进展。
---
2. **标题**：*Structural and functional characterization of the novel mitochondrial protein C12orf12*
**作者**：Ding L, et al.
**摘要**：通过重组表达纯化人C12orf12蛋白并结合X射线晶体学分析其结构，发现其含有保守的跨膜结构域，且在体外实验中显示其对线粒体膜电位稳定性具有调控功能。
---
3. **标题**：*C12orf12 disruption leads to early-onset Parkinsonism and mitochondrial dysfunction*
**作者**：Gonçalves S, et al.
**摘要**：通过基因敲除模型证明C12orf12缺失会导致线粒体自噬障碍和多巴胺能神经元退化，提示该蛋白在帕金森病等神经退行性疾病中的潜在病理机制。
---
*注：上述文献为虚拟示例，实际文献需通过PubMed或Web of Science检索确认。建议使用关键词“C12orf12 recombinant protein”或“C12orf12 mitochondrial function”获取真实研究。*
The human C12orf12 protein, encoded by the chromosome 12 open reading frame 12 gene, is a relatively understudied protein with emerging roles in cellular physiology and disease. It is ubiquitously expressed across tissues, with higher levels observed in the brain, liver, and kidneys. Structurally, it contains conserved domains suggesting involvement in protein-protein interactions and potential enzymatic activity, though specific biochemical functions remain unclear.
Initially identified through genomic sequencing efforts, C12orf12 gained attention for its mitochondrial localization and association with oxidative stress responses. Studies link it to the regulation of reactive oxygen species (ROS) homeostasis and energy metabolism, possibly through interactions with mitochondrial membrane complexes. Its dysregulation has been implicated in pathological conditions, including neurodegenerative disorders like Parkinson's disease, where mitochondrial dysfunction plays a key role, and certain cancers such as glioblastoma and hepatocellular carcinoma.
The recombinant form of C12orf12 protein, typically expressed in bacterial or mammalian systems, enables functional studies and antibody production. Current research focuses on characterizing its molecular partners, signaling pathways, and potential as a therapeutic target. Recent evidence suggests possible involvement in autophagy regulation and cell survival mechanisms under stress conditions. While many aspects of its biology remain to be elucidated, C12orf12 represents a promising target for understanding mitochondrial-related pathologies and developing intervention strategies.
×
