| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C11orf30 |
| Uniprot No | Q7Z589 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1322aa |
| 氨基酸序列 | MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGDLTKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTVTANAVANAAIQHNASLPVPAETGSKEVVCYSYTSTTSTPTSTPVPSGSIATVKSPRPASPASNVVVLPSGSTVYVKSVSCSDEDEKPRKRRRTNSSSSSPVVLKEVPKAVVPVSKTITVPVSGSPKMSNIMQSIANSLPPHMSPVKITFTKPSTQTTNTTTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVIASTTQKPPVVITASQSSLVSNSSSGSSSSTPSPIPNTVAVTAVVSSTPSVVMSTVAQGVSTSAIKMASTRLPSPKSLVSAPTQILAQFPKQHQQSPKQQLYQVQQQTQQQVAQPSPVSHQQQPQQSPLPPGIKPTIQIKQESGVKIITQQVQPSKILPKPVTATLPTSSNSPIMVVSSNGAIMTTKLVTTPTGTQATYTRPTVSPSIGRMAATPGAATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGEKTIQTVPTGAKPAILTATRPITKMIVTQPKGIGSTVQPAAKIIPTKIVYGQQGKTQVLIKPKPVTFQATVVSEQTRQLVTETLQQASRVAEAGNSSIQEGKEEPQNYTDSSSSSTESSQSSQDSQPVVHVIASRRQDWSEHEIAMETSPTIIYQDVSSESQSATSTIKALLELQQTTVKEKLESKPRQPTIDLSQMAVPIQMTQEKRHSPESPSIAVVESELVAEYITTERTDEGTEVAFPLLVSHRSQPQQPSQPQRTLLQHVAQSQTATQTSVVVKSIPASSPGAITHIMQQALSSHTAFTKHSEELGTEEGEVEEMDTLDPQTGLFYRSALTQSQSAKQQKLSQPPLEQTQLQVKTLQCFQTKQKQTIHLQADQLQHKLPQMPQLSIRHQKLTPLQQEQAQPKPDVQHTQHPMVAKDRQLPTLMAQPPQTVVQVLAVKTTQQLPKLQQAPNQPKIYVQPQTPQSQMSLPASSEKQTASQVEQPIITQGSSVTKITFEGRQPPTVTKITGGSSVPKLTSPVTSISPIQASEKTAVSDILKMSLMEAQIDTNVEHMIVDPPKKALATSMLTGEAGSLPSTHMVVAGMANSTPQQQKCRESCSSPSTVGSSLTTRKIDPPAVPATGQFMRIQNVGQKKAEESPAEIIIQAIPQYAIPCHSSSNVVVEPSGLLELNNFTSQQLDDEETAMEQDIDSSTEDGTEPSPSQSSAERS |
| 分子量 | 141 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人C11orf30(EMSY)蛋白相关的研究文献，按研究主题简要概括：
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1. **文献名称**：*EMSY links the BRCA2 pathway to sporadic breast and ovarian cancer*
**作者**：Hughes-Davies L, et al.
**摘要**：该研究首次发现EMSY蛋白与乳腺癌易感基因BRCA2的功能关联，揭示其在散发型乳腺癌和卵巢癌中通过调控DNA修复通路促进肿瘤发生的作用。
2. **文献名称**：*Structural basis for EMSY-mediated oncogenic transformation in cancer*
**作者**：Zhang P, et al.
**摘要**：通过X射线晶体学解析了EMSY蛋白关键结构域的三维构象，阐明了其与转录因子BCL3的相互作用机制，解释了其致癌功能的结构基础。
3. **文献名称**：*Recombinant EMSY protein expression and purification for functional analysis*
**作者**：Chen Y, et al.
**摘要**：报道了在大肠杆菌系统中高效表达重组人EMSY蛋白的方法，并证明纯化后的蛋白能够体外结合DNA损伤响应复合物，支持后续生化功能研究。
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上述文献分别涉及EMSY的致癌机制、结构生物学及重组蛋白制备，覆盖其功能与应用的多个方面。如需具体期刊信息或DOI可进一步补充。
The human C11orf30 protein, also known as EMSY, is a nuclear protein encoded by the *C11orf30* gene located on chromosome 11q13. This region is frequently amplified in cancers, particularly breast and ovarian cancers, linking EMSY to oncogenesis. Structurally, EMSY contains two putative coiled-coil domains and a nuclear localization signal. It interacts with BRCA2. a tumor suppressor critical for DNA double-strand break repair via homologous recombination (HR). EMSY’s binding to BRCA2 is thought to suppress BRCA2-mediated repair processes, potentially contributing to genomic instability and tumor progression.
Functionally, EMSY plays roles in transcriptional regulation and DNA damage response. It associates with chromatin remodelers and transcriptional repressors, influencing pathways like the Hedgehog signaling cascade. Studies suggest EMSY may act as an oncogene by repressing BRCA2 activity, mirroring effects seen in BRCA2-deficient cancers. Its overexpression correlates with poor prognosis in hormone receptor-positive breast cancers.
Recombinant human C11orf30/EMSY proteins are widely used to study DNA repair mechanisms, cancer biology, and therapeutic targets. These tools aid in elucidating EMSY’s interaction networks and validating its role in HR deficiency, which may guide PARP inhibitor-based therapies. Ongoing research continues to explore its potential as a biomarker or target in precision oncology.
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