| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMLG |
| Uniprot No | P49069 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMESMAVATDGGERPGVPAGSGLSASQR RAELRRRKLLMNSEQRINRIMGFHRPGSGAEEESQTKSKQQDSDKLNSLS VPSVSKRVVLGDSVSTGTTDQQGGVAEVKGTQLGDKLDSFIKPPECSSDV NLELRQRNRGDLTADSVQRGSRHGLEQYLSRFEEAMKLRKQLISEKPSQE DGNTTEEFDSFR |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CAMLG重组蛋白的参考文献及其摘要概括(注：文献为示例性内容，实际引用需核实准确性)：
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1. **文献名称**: *"Recombinant CAMLG Expression in Mammalian Cells Reveals Its Role in ER Calcium Homeostasis"*
**作者**: Thompson, J. et al. (2017)
**摘要**: 该研究通过哺乳动物表达系统成功重组表达了CAMLG蛋白，并证明其通过调节内质网钙离子通道SERCA的活性，参与维持细胞内钙稳态，为探究CAMLG在代谢疾病中的作用提供了新依据。
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2. **文献名称**: *"Structural Characterization of CAMLG Using Cryo-EM and Functional Analysis in T-cell Signaling"*
**作者**: Li, X. & Chen, R. (2020)
**摘要**: 作者利用冷冻电镜技术解析了重组CAMLG蛋白的三维结构，结合体外功能实验发现其通过结合T细胞受体(TCR)复合物调控下游NFAT信号通路，揭示了CAMLG在免疫应答中的分子机制。
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3. **文献名称**: *"High-Yield Production of Soluble CAMLG in E. coli for Antibody Development Against Autoimmune Disorders"*
**作者**: Gupta, S. et al. (2019)
**摘要**: 研究优化了大肠杆菌表达系统，实现可溶性CAMLG重组蛋白的高效制备，并基于此开发了靶向抗体，实验显示该抗体可显著抑制自身免疫模型小鼠的炎症反应，具有潜在治疗价值。
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**备注**：若需具体文献，建议通过PubMed或Web of Science以关键词“CAMLG recombinant protein”或“CAMLG expression”检索最新研究。部分研究可能聚焦于CAMLG与内质网应激、免疫调控或癌症相关的分子机制。
CAMLG (Calcium-Modulating Ligand) is a conserved transmembrane protein primarily localized in the endoplasmic reticulum (ER) membrane. It plays a critical role in calcium homeostasis and signaling by facilitating the release of calcium ions from the ER lumen into the cytosol, a process essential for cellular functions such as gene expression, protein synthesis, and cell differentiation. CAMLG interacts with the stromal interaction molecule 1 (STIM1) to regulate store-operated calcium entry (SOCE), a mechanism that replenishes ER calcium stores and activates downstream signaling pathways. Its involvement in T-cell receptor (TCR) signaling and immune response modulation highlights its biological significance.
Recombinant CAMLG protein is produced using genetic engineering techniques, often expressed in mammalian or bacterial systems to ensure proper folding and post-translational modifications. The purified protein retains functional domains, including its N-terminal ligand-binding region and transmembrane segments, enabling in vitro studies of calcium signaling mechanisms. Researchers utilize recombinant CAMLG to investigate its role in diseases linked to calcium dysregulation, such as immunodeficiency disorders, cancer metastasis, and neurodegenerative conditions. It also serves as a tool for screening therapeutic compounds targeting calcium-dependent pathways. By providing a controlled, high-purity protein source, recombinant CAMLG advances both basic research and drug development efforts centered on calcium signaling networks.
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