| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMK2N2 |
| Uniprot No | Q96S95 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-79aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSEILPYSEDKMGRFGADPEGSDLSFS CRLQDTNSFFAGNQAKRPPKLGQIGRAKRVVIEDDRIDDVLKGMGEKPPS GV |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **CAMK2N2重组蛋白** 的3篇参考文献示例(部分内容基于真实研究概括，文献标题和作者为虚构示例)：
---
1. **文献名称**: "Recombinant CAMK2N2 Protein Inhibits Calcium/Calmodulin-Dependent Kinase II Activity in Neuronal Cells"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人源CAMK2N2蛋白，验证其通过结合CaMKII的催化结构域抑制激酶活性，并发现其可调节神经元突触可塑性，为研究神经退行性疾病提供工具。
2. **文献名称**: "Structural and Functional Characterization of CAMK2N2 as a Novel Endogenous CaMKII Antagonist"
**作者**: Wang Y, et al.
**摘要**: 通过X射线晶体学解析了重组CAMK2N2蛋白的结构，揭示其与CaMKII的相互作用界面，并证明其在心脏和脑组织中通过调控CaMKII信号通路影响细胞凋亡和炎症反应。
3. **文献名称**: "Development of a High-Yield CAMK2N2 Recombinant Protein Production System for Therapeutic Screening"
**作者**: Kim S, et al.
**摘要**: 开发了一种基于哺乳动物细胞的重组CAMK2N2蛋白高效表达系统，优化了纯化工艺并获得高纯度蛋白，应用于药物筛选平台，发现其潜在抗心律失常和神经保护作用。
---
如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词 "CAMK2N2 recombinant protein" 或 "CAMK2N2 CaMKII inhibitor" 获取最新研究。
**Background of CAMK2N2 Recombinant Protein**
CAMK2N2 (Calcium/Calmodulin-Dependent Protein Kinase II Inhibitor 2) is an endogenous regulatory protein that modulates the activity of calcium/calmodulin-dependent protein kinase II (CAMKII), a multifunctional serine/threonine kinase critical in calcium signaling pathways. CAMKII is involved in diverse cellular processes, including synaptic plasticity, memory formation, and gene regulation. CAMK2N2 acts as a natural inhibitor by binding to CAMKII, preventing its autophosphorylation and subsequent activation, thereby fine-tuning calcium-dependent signaling cascades.
Structurally, CAMK2N2 contains conserved domains that facilitate interaction with CAMKII isoforms. Its expression is particularly notable in the brain, suggesting a role in neuronal homeostasis. Dysregulation of CAMK2N2 has been implicated in neurological disorders, such as Alzheimer’s disease, where aberrant CAMKII activity contributes to synaptic dysfunction. Additionally, CAMK2N2 has been studied in cancer, cardiovascular diseases, and metabolic syndromes, highlighting its broad regulatory influence.
Recombinant CAMK2N2 protein is produced using expression systems like *E. coli* or mammalian cell lines, ensuring high purity and bioactivity. This engineered protein enables researchers to study CAMKII inhibition mechanisms, screen therapeutic compounds, or explore disease pathways in vitro. Its application extends to elucidating calcium signaling dynamics and developing targeted therapies for conditions linked to CAMKII hyperactivity. As a research tool, CAMK2N2 recombinant protein bridges molecular insights into calcium-dependent regulation with translational biomedical investigations.
×
