| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/300 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KOX4; Zinc finger protein 7; Zinc finger protein HF.16; Zinc finger protein KOX4 |
| Entrez GeneID | 7553; |
| WB Predicted band size | 77kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ZNF7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于ZNF7抗体的模拟参考文献示例(注：以下内容为虚构，实际文献需通过学术数据库检索)：
1. **文献名称**：*"Development and Application of a ZNF7-Specific Monoclonal Antibody for Epigenetic Studies"*
**作者**：Smith A.B., et al.
**摘要**：本研究报道了一种新型ZNF7单克隆抗体的开发，验证了其在Western blot和免疫荧光中的特异性。利用该抗体，作者发现ZNF7在乳腺癌细胞中与Polycomb抑制复合体(PRC2)共定位，提示其在基因沉默和染色质重塑中的潜在作用。
2. **文献名称**：*"ZNF7 Knockdown via CRISPR/Cas9 and Antibody-Based Validation in Neural Development Models"*
**作者**：Chen L., et al.
**摘要**：通过CRISPR/Cas9技术敲低ZNF7基因，并结合特异性抗体验证，发现ZNF7缺失导致神经干细胞分化异常。研究表明ZNF7通过调控KLF4转录因子表达影响神经发育通路。
3. **文献名称**：*"A Pan-KRAB-ZFP Antibody Panel Reveals ZNF7 as a Metastasis Suppressor in Colorectal Cancer"*
**作者**：Gupta R., et al.
**摘要**：通过KRAB锌指蛋白(ZFP)抗体库筛选，发现ZNF7在结直肠癌组织中表达下调。功能实验表明，ZNF7抗体染色强度与患者预后呈正相关，其过表达可抑制肿瘤细胞迁移和侵袭。
4. **文献名称**：*"ZNF7 Antibody-Based ChIP-Seq Analysis Uncovers Its Role in Genomic Stability"*
**作者**：Wang X., et al.
**摘要**：利用ZNF7特异性抗体进行染色质免疫沉淀测序(ChIP-seq)，发现ZNF7结合于端粒附近重复序列区域，参与维持基因组稳定性，其缺失可能导致DNA复制应激和染色体断裂。
**提示**：实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词：ZNF7 antibody, ZNF7 function)。
The zinc finger protein 7 (ZNF7) antibody is a tool used to study the ZNF7 protein, a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. ZNF7 contains multiple C2H2-type zinc finger motifs, which mediate sequence-specific DNA binding, and is implicated in transcriptional regulation, chromatin remodeling, and epigenetic modifications. It is thought to participate in developmental processes, cellular differentiation, and tumorigenesis, though its precise biological roles remain under investigation.
ZNF7 antibodies are typically produced in immunized hosts (e.g., rabbits or mice) using recombinant ZNF7 fragments or synthetic peptides as antigens. These antibodies enable researchers to detect ZNF7 expression in various experimental applications, including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Validation often involves confirming specificity via knockdown/knockout cell lines or blocking assays.
Research utilizing ZNF7 antibodies has explored its potential involvement in cancer pathways, stem cell regulation, and interactions with co-repressor complexes. Dysregulation of ZNF7 has been preliminarily linked to certain cancers, though mechanistic insights are still emerging. Commercial ZNF7 antibodies are available, but performance may vary, necessitating careful validation for experimental reproducibility.
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