| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NFIA; KIAA1439; Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein; NFIB; Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/B; NF-I/B; NFI-B; TGGCA-binding protein; NFIC; NFI; Nuclear factor 1 C-type; NF1-C; Nuclear factor 1/C; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/C; NF-I/C; NFI-C; TGGCA-binding protein; NFIX; Nuclear factor 1 X-type; NF1-X; Nuclear factor 1/X; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/X; NF-I/X; NFI-X; TGGCA-binding protein |
| Entrez GeneID | 4774; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NFIA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是三篇关于NFIA抗体的参考文献，包含文献名称、作者及简要摘要内容：
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1. **文献名称**: *Nuclear Factor I/A Regulates Stem Cell Proliferation in the Adult Brain*
**作者**: Piper M. et al. (2019)
**摘要**: 本研究利用NFIA抗体进行免疫荧光染色，发现NFIA在成年小鼠海马区神经干细胞增殖中起关键作用。抗体特异性验证显示其在神经前体细胞核内高表达，并调控细胞周期相关基因。
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2. **文献名称**: *NFIA-Mediated Gene Regulation in Kidney Development*
**作者**: Chen H. et al. (2020)
**摘要**: 通过Western blot和ChIP-seq实验结合NFIA抗体，作者揭示了NFIA在胚胎肾脏发育中调控输尿管芽分支形态发生的作用，并鉴定出多个下游靶基因(如Pax2和Wnt11)。
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3. **文献名称**: *Cross-Species Analysis of NFIA in Glial Cell Differentiation*
**作者**: Smith J.R. & Lee Y. (2021)
**摘要**: 该研究比较了小鼠和人类少突胶质细胞分化过程中NFIA的表达差异，利用NFIA抗体进行免疫组化分析，发现NFIA通过抑制神经元分化基因促进胶质细胞命运决定。
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**备注**：以上文献为示例，实际引用时需根据具体研究补充完整信息(期刊名称、卷号、页码等)。建议通过PubMed或Google Scholar以关键词“NFIA antibody” + 研究领域(如神经发育、癌症等)检索最新文献。
The NFIA (Nuclear Factor I/A) antibody is a crucial tool for studying the NFI family of transcription factors, which play pivotal roles in regulating gene expression during development and tissue homeostasis. NFIA, a member of the NFI family (NFIA, NFIB, NFIC, NFIX), binds to palindromic DNA sequences to modulate transcription, impacting processes like chromatin remodeling, cell differentiation, and organogenesis. NFIA is particularly vital in central nervous system development, gliogenesis, and the maturation of organs such as the kidneys and skeletal system.
Antibodies targeting NFIA enable researchers to detect and quantify NFIA protein expression, localization, and interactions in various biological contexts. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies help elucidate NFIA's role in developmental pathways, disease mechanisms (e.g., brain malformations, cancer), and its interplay with signaling molecules like TGF-β or Sonic Hedgehog.
Commercial NFIA antibodies are typically raised in hosts like rabbits or mice, using immunogens derived from specific regions of the human or murine NFIA protein. Validation often includes knockout cell controls to confirm specificity. Researchers must consider species reactivity, cross-reactivity with other NFI members, and application-specific validation data when selecting an NFIA antibody. Its utility spans developmental biology, neurobiology, and cancer research, making it a key reagent in understanding transcriptional regulation networks.
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