| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DUSP1; CL100; MKP1; PTPN10; VH1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; DUSP4; MKP2; VH2; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH2; Mitogen-activated protein kinase phosphatase 2; MAP kinase phosphatase 2; MKP-2 |
| Entrez GeneID | 1843; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DUSP1/4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于DUSP1/4抗体的参考文献，信息简明概括：
1. **文献名称**: "DUSP1 modulates the differentiation and activation of human dendritic cells induced by Toll-like receptor agonists"
**作者**: Hammer M, et al.
**摘要**: 本研究使用DUSP1特异性抗体(Western Blot验证)证明DUSP1通过调控MAPK信号通路，影响树突细胞分化与炎症因子分泌，提示其在免疫调节中的关键作用。
2. **文献名称**: "Selective role of DUSP4 in regulating inflammatory arthritis via ERK and JNK pathways"
**作者**: Zhang Y, et al.
**摘要**: 通过DUSP4抗体(免疫组化验证)发现，DUSP4在小鼠关节炎模型中特异性抑制ERK/JNK磷酸化，缓解关节炎症，表明其作为治疗类风湿性关节炎的潜在靶点。
3. **文献名称**: "Antibody-based profiling of DUSP1 and DUSP4 in tumor microenvironment"
**作者**: Lee S, et al.
**摘要**: 利用商业化DUSP1/4抗体(验证于多种癌细胞系)，研究显示二者在肿瘤基质细胞中差异表达，且与患者预后相关，提示其作为癌症免疫微环境标志物的潜力。
如需具体实验细节(如抗体品牌/货号)，可进一步补充说明。
DUSP1 (dual specificity phosphatase 1) and DUSP4 (dual specificity phosphatase 4), also known as MAP kinase phosphatases MKP-1 and MKP-2. are members of the dual-specificity phosphatase family. They regulate mitogen-activated protein kinase (MAPK) signaling pathways by dephosphorylating and inactivating MAP kinases such as ERK, JNK, and p38. This modulation influences cellular processes including proliferation, differentiation, apoptosis, and stress responses. DUSP1 is stress-inducible and plays roles in immune regulation, cancer progression, and metabolic diseases, while DUSP4 is implicated in controlling cytokine signaling, neuronal development, and tumor suppression.
Antibodies targeting DUSP1/4 are essential tools for studying their expression, localization, and function in biological systems. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cultured cells under various conditions (e.g., oxidative stress, inflammation, or drug treatment). These antibodies help researchers investigate how DUSP1/4 expression correlates with diseases such as cancer (e.g., chemoresistance linked to DUSP1 upregulation), autoimmune disorders, or neurodegenerative conditions. Validation of antibody specificity is critical, often involving knockout controls or siRNA-mediated knockdown. Cross-reactivity with other DUSP family members should be ruled out due to structural similarities. Commercial antibodies are typically raised against unique epitopes in human or mouse DUSP1/4. with applications spanning basic research and preclinical studies.
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