| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FKHL12; FREAC8; Forkhead box protein E3; Forkhead-related protein FKHL12; Forkhead-related transcription factor 8; FREAC-8 |
| Entrez GeneID | 2301; |
| WB Predicted band size | 33kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXE3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FOXE3抗体的参考文献示例(注：以下为假设性文献，仅供参考格式)：
1. **文献名称**: *FOXE3 mutations and anterior segment dysgenesis in humans*
**作者**: Semina EV, et al.
**摘要**: 该研究通过免疫组化技术使用FOXE3抗体，揭示了FOXE3蛋白在胚胎眼晶状体及角膜发育中的表达模式，并发现其突变与人类前节发育异常相关。
2. **文献名称**: *Role of Foxe3 in lens epithelial cell differentiation*
**作者**: Brownell I, et al.
**摘要**: 利用FOXE3抗体进行Western blot和免疫荧光实验，证明FOXE3通过调控细胞周期蛋白参与小鼠晶状体上皮细胞的分化过程，缺失导致先天性白内障。
3. **文献名称**: *FOXE3 as a transcriptional regulator in ocular development*
**作者**: Medina-Martinez O, Jamrich M
**摘要**: 研究采用FOXE3抗体进行染色质免疫沉淀(ChIP)，阐明FOXE3直接调控晶状体特异性基因的转录机制，并确认其在胚胎眼发育中的关键作用。
4. **文献名称**: *Expression analysis of Foxe3 in zebrafish models*
**作者**: Shirai K, et al.
**摘要**: 通过斑马鱼模型结合FOXE3抗体的免疫组织化学分析，发现Foxe3在视泡形成早期高表达，其缺失导致视网膜结构异常，提示跨物种保守功能。
**注意**：以上文献信息为示例性质，实际引用时需查询真实数据库(如PubMed、Google Scholar)获取准确信息。
The FOXE3 antibody is a research tool used to detect and study the FOXE3 protein, a member of the forkhead box (FOX) family of transcription factors. FOXE3 plays a critical role in ocular development, particularly in lens and corneal formation during embryogenesis. It regulates gene expression by binding to DNA through its conserved forkhead domain, influencing cellular differentiation and tissue morphogenesis. Mutations in the FOXE3 gene are associated with congenital eye disorders, including primary aphakia, microphthalmia, and anterior segment dysgenesis, highlighting its importance in eye development.
In research, FOXE3 antibodies are primarily utilized in techniques like immunohistochemistry, Western blotting, and immunofluorescence to localize and quantify FOXE3 expression in tissues, especially ocular samples. These studies help elucidate FOXE3's role in developmental pathways and disease mechanisms. For instance, FOXE3 is expressed in lens epithelial cells, where it suppresses cell proliferation and promotes fiber cell differentiation. Dysregulation of FOXE3 has been linked to pathological conditions, making its antibody valuable for investigating developmental defects and potential therapeutic targets.
FOXE3 antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides derived from the protein's conserved regions. Validation includes specificity tests (e.g., knockout cell controls) and functional assays to ensure biological relevance. Their application extends to both basic developmental biology and clinical research on inherited eye diseases.
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