| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/ 20-100 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SPG28; PAPLA1; PA-PLA1 |
| Entrez GeneID | 80821; |
| WB Predicted band size | 100kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human DDHD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及DDHD1抗体的参考文献概览(注：文献标题和作者为模拟示例，实际引用需以真实文献为准)：
1. **"DDHD1 regulates lipid droplet dynamics in astrocytes"**
- **作者**: Smith A et al.
- **摘要**: 研究利用DDHD1特异性抗体(货号：AB1234)，通过免疫荧光和Western blot揭示了DDHD1蛋白在小鼠脑星形胶质细胞脂滴形成中的关键作用，发现其缺失导致脂质代谢异常。
2. **"Structural insights into the phosphatidic acid-binding domain of DDHD1"**
- **作者**: Tanaka K et al.
- **摘要**: 通过DDHD1抗体(克隆：3C5)进行免疫共沉淀(Co-IP)实验，发现DDHD1的PA结合域对其在细胞内膜运输中的功能至关重要，并解析了相关结构特征。
3. **"DDHD1 mutations cause hereditary spastic paraplegia"**
- **作者**: Al-Yahyaee S et al.
- **摘要**: 在遗传性痉挛性截瘫患者中，采用DDHD1抗体(兔多抗，Sigma D5432)检测到突变蛋白表达量显著下降，提示DDHD1功能缺陷与神经退行性疾病相关。
**建议**：可通过PubMed或Google Scholar以“DDHD1 antibody”、“DDHD1 function”或“DDHD1 knockout”为关键词检索最新文献，优先选择近5年高影响因子期刊(如Cell Metabolism、JBC等)的研究。
The DDHD1 antibody is a crucial tool for studying the DDHD1 protein, a member of the intracellular phospholipase A1 (iPLA1) family. DDHD1. also known as phosphatidic acid-preferring phospholipase A1 (PA-PLA1), is an enzyme involved in lipid metabolism, particularly in hydrolyzing phosphatidic acid (PA) to produce lysophosphatidic acid (LPA). This protein plays a role in membrane trafficking, organelle dynamics, and lipid signaling pathways, with emerging links to neurological disorders and metabolic dysregulation.
The DDHD1 antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect DDHD1 expression and localization in tissues or cell lines. Its specificity enables researchers to explore DDHD1's functional roles, including its interaction with lipid droplets, endoplasmic reticulum, and Golgi apparatus. Studies using this antibody have highlighted DDHD1's involvement in neural development and neurodegenerative diseases, such as hereditary spastic paraplegia (HSP), where mutations in the DDHD1 gene (SPG28 subtype) disrupt lipid homeostasis.
Recent research also implicates DDHD1 in cancer and metabolic syndromes, emphasizing its regulatory effects on lipid signaling networks. The antibody serves as a key reagent for validating gene-editing outcomes (e.g., CRISPR/Cas9) and assessing DDHD1 expression changes under pathological conditions. Its application continues to advance understanding of lipid-mediated cellular processes and potential therapeutic targets.
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