| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IMB3; Pse1; imp5; KPNB3; RANBP5 |
| Entrez GeneID | 3843; |
| WB Predicted band size | 124kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human IPO5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于IPO5抗体的3篇参考文献概览：
1. **文献名称**：*"IPO5 mediates transport of tumor suppressors into the nucleus and its overexpression correlates with poor prognosis in lung cancer"*
**作者**：Li Y. et al.
**摘要**：该研究开发了一种特异性IPO5抗体，用于检测肺癌组织中IPO5蛋白表达水平。发现IPO5通过转运p53等抑癌蛋白入核，其高表达与患者预后不良相关，为肺癌治疗提供了潜在靶点。
2. **文献名称**：*"Characterization of a monoclonal antibody targeting Importin-5 for inhibition of viral replication"*
**作者**：Smith J. et al.
**摘要**：研究团队制备了抗IPO5的单克隆抗体，证实其可阻断HIV-1病毒整合前复合体的核转运。抗体在细胞模型中有效抑制病毒复制，提示IPO5抗体在抗病毒治疗中的应用潜力。
3. **文献名称**：*"Structural analysis of Importin-5 using cryo-EM reveals cargo-binding domain epitopes"*
**作者**：Tanaka R. et al.
**摘要**：通过冷冻电镜解析IPO5结构，并利用特异性抗体定位其货物结合域。研究发现抗体结合可竞争性抑制IPO5与核定位信号(NLS)的结合，为设计核转运调控工具提供了结构基础。
注：上述文献为虚拟示例，实际研究中建议通过PubMed或Web of Science以“IPO5 antibody”为关键词检索最新实证论文。
The IPO5 antibody targets Importin-5 (IPO5), a member of the importin-β family of nuclear transport receptors. IPO5. also known as RanBP5 or KPNB3. facilitates nucleocytoplasmic transport by shuttling cargoes between the cytoplasm and nucleus. It specifically recognizes substrates containing a PY-NLS (proline-tyrosine nuclear localization signal), including critical molecules like certain RNA-binding proteins, transcription factors, and pre-mRNA splicing factors. This transport mechanism is essential for regulating gene expression, RNA processing, and cellular signaling pathways.
IPO5 antibodies are widely used in research to study nuclear transport dynamics, subcellular localization of cargo proteins, and IPO5’s role in diseases. For instance, dysregulated IPO5 expression has been linked to cancer progression and viral infections, where pathogens hijack nuclear import machinery. These antibodies are validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry, enabling visualization of IPO5 distribution in cells and tissues. Many commercially available IPO5 antibodies are raised against specific epitopes, ensuring high specificity and affinity. Their utility extends to functional studies, including siRNA-mediated knockdown experiments to explore IPO5’s biological impact. Overall, IPO5 antibodies serve as vital tools for dissecting nuclear transport mechanisms and their implications in health and disease.
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