| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DIPP2; HDCMB47P; DIPP2beta; DIPP2alpha |
| Entrez GeneID | 11163; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein of human NUDT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NUDT4抗体的3篇参考文献示例(注：文献为虚拟示例，实际引用请查询权威数据库)：
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1. **文献名称**：*Characterization of NUDT4 Antibody Specificity in Human Tissues*
**作者**：Smith J, et al.
**摘要**：本研究验证了一种新型NUDT4抗体的特异性，通过Western blot和免疫组化证实其在多种人类组织(如脑、肝脏)中的表达模式，并发现NUDT4蛋白在神经元中的高表达可能与RNA代谢调控相关。
2. **文献名称**：*NUDT4 as a Novel Biomarker in Glioblastoma: Insights from Antibody-Based Profiling*
**作者**：Lee H, et al.
**摘要**：利用NUDT4特异性抗体分析胶质母细胞瘤样本，发现其表达水平与肿瘤侵袭性呈负相关，提示NUDT4可能通过调控氧化应激通路抑制癌细胞增殖。
3. **文献名称**：*Development of a Monoclonal Antibody Targeting NUDT4 for Metabolic Studies*
**作者**：Wang Y, et al.
**摘要**：报道了一种高亲和力单克隆抗体的开发，通过免疫沉淀和质谱分析揭示NUDT4与AMPK通路的相互作用，为研究其在能量代谢中的功能提供了工具。
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**提示**：实际研究中，建议通过PubMed或Google Scholar检索“NUDT4 antibody”或“NUDT4 immunodetection”获取最新文献，重点关注抗体验证方法(如KO细胞系验证)及疾病关联研究。
The NUDT4 antibody is a crucial tool for studying the NUDT4 protein, a member of the NUDIX hydrolase family involved in nucleotide metabolism and redox homeostasis. NUDT4. also known as DIPP1 (diphosphoinositol polyphosphate phosphohydrolase 1), primarily hydrolyzes diadenosine polyphosphates (e.g., Ap6A) and diphosphoinositol polyphosphates (e.g., PP-IP5), regulating cellular signaling and stress responses. Its role in modulating inositol pyrophosphate levels links it to processes like DNA repair, apoptosis, and energy sensing.
NUDT4 antibodies are widely used in research to detect protein expression, localization, and function via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are critical for exploring NUDT4's involvement in diseases, including cancer (where dysregulation may affect cell proliferation) and neurodegenerative disorders (linked to oxidative stress).
Structurally, NUDT4 contains a conserved NUDIX motif essential for catalytic activity. Antibodies targeting specific epitopes (e.g., N-terminal or catalytic domains) help distinguish NUDT4 from homologous NUDIX proteins. Validation via knockout controls or siRNA knockdown is recommended to ensure specificity, as cross-reactivity with related enzymes (e.g., NUDT3 or NUDT10) may occur. Commercial NUDT4 antibodies are typically raised in rabbits or mice, with monoclonal variants offering higher specificity. Researchers should optimize protocols for tissue or cell types, as expression levels vary across biological systems. Overall, NUDT4 antibodies advance understanding of nucleotide signaling networks and their pathophysiological implications.
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