| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GYPA; GPA; Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD235a |
| Entrez GeneID | 2993; |
| WB Predicted band size | 16kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the Internal region of human CD235a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于CD235a抗体的模拟参考文献示例(非真实文献，仅供参考格式)：
1. **文献名称**: *CD235a as a Specific Marker for Erythroid Differentiation in Human Hematopoietic Stem Cells*
**作者**: Tanaka M, et al.
**摘要**: 研究利用CD235a抗体通过流式细胞术追踪造血干细胞向红细胞系分化的过程，证实CD235a在红细胞成熟晚期特异性表达，可作为红细胞分化的可靠标志物。
2. **文献名称**: *Utility of CD235a Antibody in Diagnosing Myelodysplastic Syndromes (MDS)*
**作者**: Chen L, et al.
**摘要**: 通过CD235a抗体检测骨髓样本中红细胞前体细胞的异常表面抗原表达，发现CD235a低表达与MDS患者的疾病严重程度和预后不良显著相关。
3. **文献名称**: *CD235a-Mediated Plasmodium falciparum Invasion of Human Erythrocytes*
**作者**: Kumar R, et al.
**摘要**: 研究利用CD235a抗体阻断实验，证明疟原虫(*Plasmodium*)通过结合红细胞表面CD235a糖蛋白入侵宿主细胞，为抗疟药物靶点开发提供依据。
4. **文献名称**: *Flow Cytometric Analysis of CD235a in Hereditary Spherocytosis*
**作者**: Silva E, et al.
**摘要**: 采用CD235a抗体结合膜蛋白定量分析，发现遗传性球形红细胞增多症患者红细胞表面CD235a表达显著降低，提示其可用于辅助诊断和病理机制研究。
(注：以上内容为模拟生成，实际文献需通过学术数据库检索确认。)
CD235a, also known as glycophorin A (GPA), is a transmembrane sialoglycoprotein predominantly expressed on the surface of human erythrocytes and their precursors. As a key component of the red blood cell (RBC) membrane, it plays a critical role in maintaining cellular structural integrity, flexibility, and antigenic properties. The CD235a antigen carries the M/N blood group determinants, making it clinically relevant in transfusion medicine and hemolytic disease studies.
CD235a-specific monoclonal antibodies are widely used in biomedical research and diagnostics. In flow cytometry, these antibodies enable precise identification and enumeration of erythroid cells, helping distinguish mature RBCs from nucleated precursors in bone marrow or peripheral blood samples. This is particularly valuable in diagnosing disorders like aplastic anemia, myelodysplastic syndromes, and leukemia, where abnormal erythroid maturation may occur. Additionally, CD235a antibodies are employed in immunoassays to detect fetal RBCs in maternal circulation, aiding in the management of hemolytic disease of the fetus and newborn.
In research contexts, CD235a serves as a marker for studying erythropoiesis, RBC membrane disorders, and malaria pathogenesis, as Plasmodium falciparum parasites utilize glycophorin A for erythrocyte invasion. The antibody's specificity and reliability have cemented its role as an essential tool in hematology and immunology.
×
