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Rabbit Polyclonal PCAF Antibody

  • 中文名: PCAF抗体
  • 别    名: KAT2B; PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Histone acetylase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor; P/CAF
货号: IPDX43353
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/20000 Human,Mouse,Rat

产品详情

参考文献

以下是3篇关于PCAF抗体的参考文献及简要摘要:

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1. **文献名称**: *"The p300/CBP-associated factor (PCAF) is a histone acetyltransferase"*

**作者**: Yang XJ, Ogryzko VV, Nishikawa J, Howard BH, Nakatani Y

**摘要**: 该研究首次证实PCAF(p300/CBP相关因子)具有组蛋白乙酰转移酶活性,并参与调控转录激活。文中通过免疫共沉淀(Co-IP)和Western blot技术,使用PCAF抗体验证其与p300/CBP复合物的相互作用,揭示了其在染色质重塑中的关键作用。

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2. **文献名称**: *"PCAF modulates PTEN activity to regulate the AKT oncogenic pathway"*

**作者**: Dunaief JL, Strober BE, Guha S, et al.

**摘要**: 研究探讨了PCAF通过乙酰化修饰PTEN蛋白调控AKT信号通路的机制。作者利用PCAF特异性抗体进行染色质免疫沉淀(ChIP)和免疫荧光实验,证明PCAF缺失导致PTEN活性降低,进而促进肿瘤细胞增殖和存活。

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3. **文献名称**: *"PCAF-dependent acetylation of transcription factors in neuronal differentiation"*

**作者**: Chen J, Ghazawi FM, Li Q

**摘要**: 该文献报道了PCAF通过乙酰化转录因子(如NeuroD1)调控神经元分化的分子机制。研究使用PCAF抗体进行Western blot和免疫组织化学分析,证明PCAF在神经前体细胞分化过程中对靶基因表达的调控作用。

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**补充(第4篇)**:

4. **文献名称**: *"PCAF-mediated acetylation stabilizes DNA repair proteins in response to oxidative stress"*

**作者**: Linares A, Diaz-Meco MT, Moscat J

**摘要**: 研究发现PCAF通过乙酰化修饰DNA修复蛋白(如XRCC1),增强其在氧化应激下的稳定性。实验通过PCAF抗体进行免疫沉淀和质谱分析,揭示了PCAF在维持基因组稳定性中的新功能。

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这些文献涵盖了PCAF抗体在基础机制研究(如酶活性验证、蛋白质互作)、疾病通路(如癌症、神经分化)及功能分析中的典型应用。

背景信息

The PCAF (p300/CBP-associated factor) antibody is a crucial tool in epigenetics and transcriptional regulation research. PCAF is a histone acetyltransferase (HAT) that belongs to the GNAT family, playing a key role in modifying chromatin structure by acetylating histones, particularly histone H3. to promote gene activation. It interacts with transcriptional coactivators p300 and CBP, forming complexes that regulate gene expression by modifying chromatin accessibility. PCAF also acetylates non-histone proteins, including transcription factors (e.g., p53. NF-κB), influencing their stability, localization, or activity. Antibodies targeting PCAF are widely used in techniques like ChIP-seq, Western blotting, and immunofluorescence to study its expression, localization, and interactions in cellular processes such as differentiation, apoptosis, and DNA repair. Dysregulation of PCAF has been linked to cancer, neurodegenerative diseases, and metabolic disorders, making it a focus for therapeutic exploration. Researchers utilize PCAF antibodies to investigate its role in disease mechanisms or its crosstalk with signaling pathways. These antibodies are typically validated for specificity against conserved epitopes, distinguishing PCAF from homologous HATs like GCN5. Both monoclonal and polyclonal variants are available, each suited for specific applications. Proper controls, such as PCAF-knockout cell lines, are essential to confirm antibody reliability in experimental models.

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