| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Entrez GeneID | 5893 |
| clone | 5E11B2 |
| WB Predicted band size | 46.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RAD52 (AA: 269-418) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于HSP70抗体的经典文献概览：
1. **文献名称**: "Heat shock proteins in cancer: diagnostic, prognostic, predictive, and treatment implications"
**作者**: Ciocca, D.R., Calderwood, S.K.
**摘要**: 该综述探讨了HSP70作为癌症生物标志物的潜力，指出HSP70抗体在肿瘤组织检测中可反映肿瘤侵袭性和患者预后，其过表达与化疗耐药相关。
2. **文献名称**: "HSP70 antibodies in neurodegenerative diseases: link between neurodegeneration and autoimmunity"
**作者**: Rosen, D.R., et al.
**摘要**: 研究显示，HSP70抗体在阿尔茨海默病和肌萎缩侧索硬化症(ALS)患者血清中异常升高，提示HSP70可能通过免疫交叉反应参与神经退行过程。
3. **文献名称**: "Extracellular HSP70: role in immunity and disease"
**作者**: Asea, A., et al.
**摘要**: 揭示了细胞外HSP70通过结合Toll样受体(TLR2/4)激活先天免疫反应，其特异性抗体可调控炎症信号通路，为感染和自身免疫病治疗提供新靶点。
4. **文献名称**: "Antibody specificity validation by Western blotting: guidelines for HSP70 studies"
**作者**: Kampinga, H.H., et al.
**摘要**: 提出标准化HSP70抗体验证流程，强调不同物种/亚型交叉反应性差异，建议结合敲除细胞模型确保抗体特异性，避免实验假阳性结果。
HSP70 antibodies are immunological tools used to detect and study the heat shock protein 70 (HSP70) family, a group of highly conserved molecular chaperones critical for protein homeostasis. HSP70 proteins, including constitutive (HSC70) and stress-inducible (HSP72) forms, assist in folding nascent polypeptides, preventing aggregation, and facilitating the refolding or degradation of misfolded proteins under cellular stress (e.g., heat, oxidative stress, or toxins). Their roles extend to immune regulation, apoptosis modulation, and cellular adaptation to environmental challenges.
HSP70 antibodies are widely employed in research to investigate HSP70 expression patterns in diseases. Elevated HSP70 levels are observed in cancers, neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), and inflammatory conditions, correlating with disease progression or cytoprotective responses. These antibodies are essential in techniques like Western blotting, immunohistochemistry, and flow cytometry to localize HSP70 in tissues or cells.
Clinically, HSP70 antibodies aid in biomarker discovery and therapeutic targeting. For instance, tumor-specific HSP70 overexpression is exploited in cancer immunotherapy, while autoantibodies against HSP70 are linked to autoimmune diseases. However, specificity remains a challenge due to homology among HSP70 isoforms and cross-reactivity with bacterial orthologs.
Overall, HSP70 antibodies serve as vital reagents in unraveling cellular stress responses and developing diagnostic or therapeutic strategies.
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