| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | CACYBP; S100A6BP; SIP; PNAS-107; Calcyclin-binding protein; CacyBP; hCacyBP; S100A6-binding protein; Siah-interacting protein |
| Entrez GeneID | 27101; |
| WB Predicted band size | 25kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the C-terminal region of human CacyBP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于CacyBP抗体的3篇文献示例(内容基于公开研究概括，非真实文献)：
1. **文献名称**：*CacyBP/SIP antibody detects differential expression in neuroblastoma cell differentiation*
**作者**：A. Filipek et al.
**摘要**：研究利用特异性CacyBP抗体，通过免疫印迹和免疫荧光技术，发现CacyBP在神经母细胞瘤分化过程中表达上调，并参与调节S100A6蛋白相互作用，提示其在肿瘤细胞分化中的潜在作用。
2. **文献名称**：*Development and validation of a polyclonal antibody against human CacyBP for gastric cancer studies*
**作者**：X. Wang et al.
**摘要**：报道了一种新型兔源多克隆CacyBP抗体的制备与验证，该抗体成功应用于胃癌组织芯片的免疫组化分析，证实CacyBP在胃癌组织中表达显著降低，且与患者预后相关。
3. **文献名称**：*CacyBP regulates β-catenin degradation via an antibody-based mechanistic study*
**作者**：M. Schneider et al.
**摘要**：通过CacyBP特异性抗体阻断实验，揭示CacyBP通过泛素-蛋白酶体通路促进β-catenin降解，为结直肠癌中Wnt信号异常提供机制解释。
(注：以上文献为模拟示例，实际文献需通过PubMed/Google Scholar检索关键词如“CacyBP antibody”或“CacyBP/SIP”获取。)
CacyBP (Calcyclin Binding Protein), also known as Siah-1-interacting protein (SIP), is a multifunctional protein involved in cellular processes such as proliferation, differentiation, and apoptosis. It interacts with various partners, including S100 proteins, Skp1. and the E3 ubiquitin ligase Siah-1. playing roles in ubiquitination pathways, β-catenin regulation, and stress response. CacyBP's phosphorylation status (e.g., by ERK1/2) modulates its activity and interactions, linking it to signaling cascades like the MAPK pathway.
CacyBP antibodies are essential tools for studying its expression, localization, and molecular interactions. They are generated using immunogens such as recombinant CacyBP fragments or synthetic peptides, typically in hosts like rabbits or mice. These antibodies are validated for applications including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Specificity is confirmed using knockout cell lines or siRNA-mediated knockdown controls.
Research using CacyBP antibodies has highlighted its dual roles in cancer—acting as a tumor suppressor or promoter depending on context. Dysregulated CacyBP expression is observed in cancers like neuroblastoma and colorectal carcinoma, suggesting potential diagnostic or therapeutic relevance. Challenges remain in distinguishing post-translational modifications and isoform-specific signals, emphasizing the need for well-validated antibodies. Overall, CacyBP antibodies are critical for unraveling its complex biology and translational potential.
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