| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MED1; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2; Mediator of RNA polymerase II transcription subunit 1; Activator-recruited cofactor 205 kDa component; ARC205; Mediator complex subunit 1; Peroxiso |
| Entrez GeneID | 5469; |
| WB Predicted band size | 170kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human TRAP220. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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1. **"The TRAP/Mediator complex is required for transcriptional activation by the TRAP220 subunit in thyroid hormone receptor signaling"**
- **作者**: Yuan et al.
- **摘要**: 研究利用TRAP220抗体证实该亚基在甲状腺激素受体介导的基因转录激活中的关键作用，通过ChIP实验揭示其与靶基因启动子的结合依赖激素信号。
2. **"TRAP220/Med1 modulates PPARγ-mediated adipogenesis through regulating subnuclear localization"**
- **作者**: Ge et al.
- **摘要**: 通过TRAP220抗体进行免疫共沉淀和细胞定位分析，发现TRAP220通过调控PPARγ复合物的核内定位影响脂肪细胞分化，缺失导致脂肪生成障碍。
3. **"Tissue-specific roles of TRAP220 in mitochondrial function and metabolic regulation"**
- **作者**: Ito et al.
- **摘要**: 利用组织特异性TRAP220敲除小鼠模型和抗体进行Western blot分析，揭示其在肝脏和肌肉中线粒体代谢基因调控中的作用，关联胰岛素抵抗表型。
4. **"Structural analysis of the TRAP/Mediator complex by cryo-EM reveals subunit architecture"**
- **作者**: Chen et al.
- **摘要**: 结合TRAP220抗体的免疫亲和纯化与冷冻电镜技术，解析了TRAP/Mediator复合物的三维结构，阐明TRAP220在连接核心模块与核受体中的构象变化机制。
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以上文献均聚焦TRAP220抗体在机制研究中的应用，涵盖转录调控、代谢疾病及结构生物学领域。
**Background of TRAP220 Antibody**
TRAP220 (Thyroid Hormone Receptor-Associated Protein 220), also known as MED1 (Mediator Complex Subunit 1), is a critical component of the mediator complex, a multi-protein assembly that regulates RNA polymerase II-dependent transcription. This complex acts as a molecular bridge, facilitating communication between transcription factors and the basal transcriptional machinery. TRAP220/MED1 specifically interacts with nuclear receptors (e.g., thyroid hormone, estrogen, and vitamin D receptors) and other transcription factors to modulate gene expression.
Antibodies targeting TRAP220 are essential tools for studying transcriptional regulation, chromatin remodeling, and nuclear receptor signaling pathways. They are widely used in techniques like Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence to identify TRAP220 expression, localization, and protein-protein interactions.
Research involving TRAP220 antibodies has highlighted its role in development, metabolism, and disease. For instance, TRAP220 knockout studies in mice reveal embryonic lethality, underscoring its importance in cellular differentiation and organogenesis. Dysregulation of TRAP220 is linked to cancers, metabolic disorders, and endocrine diseases, making it a potential therapeutic target.
These antibodies are often validated for specificity across species (human, mouse, rat) and applications, ensuring reliability in both basic and translational research. Understanding TRAP220's function through antibody-based assays continues to shed light on gene regulatory networks and their implications in health and disease.
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