| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme |
| Entrez GeneID | 7153; |
| WB Predicted band size | 174kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Topo IIα around the non-phosphorylation site of S1525. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3条关于Topo IIα抗体的参考文献示例(内容为虚拟生成，仅作格式参考)：
1. **"Topoisomerase IIα as a prognostic marker in breast cancer: Validation of a monoclonal antibody-based assay"**
- 作者：Smith J, et al.
- 摘要：研究验证了一种抗Topo IIα单克隆抗体在乳腺癌组织中的染色特异性，证明其表达水平与肿瘤增殖指数及患者预后显著相关，为临床分型提供依据。
2. **"Development of a novel anti-Topo IIα antibody for detecting chemotherapy resistance in ovarian cancer"**
- 作者：Chen L, et al.
- 摘要：报道了一种高灵敏度抗Topo IIα抗体的开发，通过免疫组化分析发现，Topo IIα低表达与铂类化疗耐药性相关，提示其作为耐药标志物的潜力。
3. **"Topo IIα antibody cross-reactivity analysis in human and murine models"**
- 作者：Wang Y, et al.
- 摘要：系统评估了多种商业Topo IIα抗体在人和小鼠组织中的交叉反应性，揭示了不同抗体在染色特异性上的差异，为实验选择提供指导。
注：以上为示例性内容，实际文献需通过PubMed或学术数据库检索。
The Topo IIα (DNA topoisomerase II alpha) antibody is a crucial tool in molecular and cancer research, targeting the enzyme responsible for managing DNA topology during replication and chromosome segregation. Topo IIα, encoded by the TOP2A gene, is a nuclear enzyme that resolves DNA supercoils and tangles by introducing transient double-strand breaks, particularly during the G2/M phase of the cell cycle. Its expression is tightly linked to cell proliferation, making it a biomarker for actively dividing cells.
In research, Topo IIα antibodies are widely used in techniques like immunohistochemistry (IHC) and Western blotting to assess protein levels in tissues or cell lines. Elevated Topo IIα expression is associated with high-grade malignancies, including breast, ovarian, and hematologic cancers, and correlates with aggressive tumor behavior. Clinically, it serves as a predictive marker for chemotherapeutic agents targeting Topo IIα, such as etoposide and anthracyclines, though its overexpression may also indicate resistance mechanisms.
Additionally, Topo IIα antibodies aid in studying genomic instability, as dysregulated enzyme activity can lead to chromosomal translocations or deletions. In diagnostics, they help differentiate proliferative from quiescent tumor regions, complementing markers like Ki-67. However, interpretation requires caution due to variable expression across tumor subtypes and cell cycle phases. Overall, Topo IIα antibodies bridge basic research and clinical applications, offering insights into cancer biology and therapeutic targeting.
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