Rabbit Polyclonal TRAP100 Antibody

The TRAP100 antibody targets TRAP100 (Thyroid Hormone Receptor-Associated Protein 100), a component of the TRAP/Mediator complex, a multi-subunit transcriptional coactivator critical for RNA polymerase II-mediated gene regulation. TRAP100. also known as MED24. is part of the Mediator complex that bridges transcription factors and the basal transcriptional machinery, modulating chromatin structure and enhancer-promoter interactions. It plays a key role in nuclear receptor signaling, including thyroid hormone and steroid hormone pathways, by facilitating ligand-dependent transcriptional activation. TRAP100 antibodies are essential tools for studying Mediator complex assembly, gene-specific transcriptional regulation, and its dysregulation in diseases like cancer, where Mediator subunits are frequently mutated or overexpressed. Researchers use these antibodies in techniques such as Western blotting, immunoprecipitation, and ChIP-seq to explore TRAP100’s involvement in cellular differentiation, metabolism, and oncogenesis. Studies have linked TRAP100 abnormalities to transcriptional dysfunction in tumors and developmental disorders, underscoring its biomedical relevance. The antibody’s specificity enables precise analysis of Mediator complex dynamics, aiding mechanistic insights into transcriptional control and therapeutic targeting.