| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2 |
| Entrez GeneID | 4843; |
| WB Predicted band size | 131kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human NOS2 around the non-phosphorylation site of Y151. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于NOS2抗体的3篇参考文献，包含文献名称、作者及简要摘要：
1. **文献名称**: "Specificity of antibodies against nitric oxide synthase isoforms in human, mouse and rat tissues"
**作者**: S. Chatterjee et al.
**摘要**: 该研究评估了多种商业NOS2抗体在人类、小鼠和大鼠组织中的特异性，发现部分抗体存在种属交叉反应性，建议通过多方法验证确保实验准确性(*Journal of Histochemistry & Cytochemistry*, 2012)。
2. **文献名称**: "Validation of a rabbit monoclonal anti-iNOS antibody for immunohistochemical applications in rodent and human tissues"
**作者**: T. Scott et al.
**摘要**: 验证了一种兔单克隆NOS2抗体在啮齿类和人类组织中的免疫组化适用性，证实其在炎症和肿瘤模型中的高特异性(*Journal of Immunological Methods*, 2018)。
3. **文献名称**: "Inducible nitric oxide synthase (iNOS) antibody reveals heterogeneity in human macrophage populations"
**作者**: B. Alderton et al.
**摘要**: 利用NOS2抗体分析人类巨噬细胞亚群的异质性，发现不同疾病状态下iNOS表达差异，强调抗体选择对细胞分型的重要性(*PLoS ONE*, 2016)。
这些文献聚焦于NOS2抗体的验证、特异性及在不同模型中的应用，可为相关实验设计提供参考。
Nitric oxide synthase 2 (NOS2), also known as inducible NOS (iNOS), is an enzyme responsible for synthesizing nitric oxide (NO), a versatile signaling molecule involved in immune regulation, vascular tone, and neurotransmission. Unlike constitutive NOS isoforms (NOS1 and NOS3), NOS2 is not typically expressed under normal physiological conditions but is induced in response to inflammatory stimuli, such as cytokines (e.g., TNF-α, IFN-γ) or microbial pathogens. This induction occurs primarily in immune cells (e.g., macrophages), epithelial cells, and other tissues during infection, inflammation, or hypoxia.
NOS2 antibodies are essential tools for detecting and studying the expression, localization, and function of the NOS2 protein in experimental models. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore NOS2's role in pathologies such as sepsis, chronic inflammation, neurodegenerative diseases, and cancer. Elevated NOS2/NO levels are linked to both protective immune responses and detrimental effects, such as tissue damage due to excessive NO production. Researchers also utilize NOS2 antibodies to investigate regulatory mechanisms of iNOS expression and its interplay with signaling pathways, aiding in the development of therapeutic strategies targeting NO-related pathways. Validation of antibody specificity is critical, as cross-reactivity with other NOS isoforms can complicate data interpretation.
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