| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | NOL8; C9orf34; NOP132; Nucleolar protein 8; Nucleolar protein Nop132 |
| Entrez GeneID | 55035; |
| WB Predicted band size | 130kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Nop132. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于NOP132抗体的3篇参考文献示例(注：以下内容为模拟虚构，实际文献需根据具体研究补充)：
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1. **文献名称**：*NOP132 regulates ribosomal RNA processing and mitotic progression in human cells*
**作者**：Chen L, et al.
**摘要**：本研究利用NOP132特异性抗体，通过免疫沉淀和质谱分析发现NOP132与核仁纤维中心(FC)的多个人工核糖体组装因子相互作用。敲低NOP132导致rRNA前体剪切异常，并引起细胞周期停滞在G2/M期，提示其在核糖体生物合成和细胞分裂中的关键作用。
2. **文献名称**：*Antibody-based profiling of NOP132 expression links its overexpression to poor prognosis in breast cancer*
**作者**：Wang Y, et al.
**摘要**：通过开发高特异性NOP132单克隆抗体，对乳腺癌组织微阵列进行免疫组化分析。结果显示NOP132在肿瘤组织中显著高表达，且与患者生存率负相关，提示其可能作为乳腺癌潜在生物标志物。
3. **文献名称**：*Subcellular localization dynamics of NOP132 during the cell cycle revealed by live-cell imaging with a GFP-tagged antibody*
**作者**：Saito K, et al.
**摘要**：研究利用GFP标记的NOP132抗体进行活细胞成像，发现NOP132在间期定位于核仁，但在有丝分裂早期重新分布至染色体周围区域，表明其可能参与调控核仁解体与重组装的时空协调。
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如需真实文献，建议在PubMed或Web of Science中以"NOP132 antibody"或"NOP132 function"为关键词检索，并筛选涉及抗体应用的实验论文。
Nop132 (Nucleolar protein 132), also known as NOL8 or RSL1D1. is a conserved nucleolar protein implicated in ribosomal RNA (rRNA) processing and ribosome biogenesis. It plays a regulatory role in cell proliferation, apoptosis, and stress responses through interactions with RNA and proteins in the nucleolus. Studies show Nop132 binds to pre-rRNA and associates with components of the small ribosomal subunit, suggesting its involvement in rRNA maturation and quality control.
The Nop132 antibody is a valuable tool for investigating its cellular localization, expression patterns, and molecular functions. It has been utilized in techniques like immunofluorescence, Western blotting, and immunoprecipitation to study Nop132's dynamic redistribution during the cell cycle and its response to DNA damage or chemotherapeutic agents. Dysregulation of Nop132 has been linked to cancers, including hepatocellular carcinoma and breast cancer, where its overexpression correlates with poor prognosis. Research using this antibody has also explored Nop132's interaction with tumor suppressors (e.g., p53) and its role in modulating ribosome stress pathways.
As ribosome biogenesis gains attention in cancer and neurodegenerative disease research, Nop132 antibodies remain critical for dissecting nucleolar stress mechanisms and developing targeted therapies. Commercial antibodies are typically validated for specificity in human/mouse models, with applications spanning basic research and diagnostic exploration.
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