| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NFYC; Nuclear transcription factor Y subunit gamma; CAAT box DNA-binding protein subunit C; Nuclear transcription factor Y subunit C; NF-YC; Transactivator HSM-1/2 |
| Entrez GeneID | 4802; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the N-terminal region of human NF-YC. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于NF-YC抗体的3篇参考文献及其简要摘要：
1. **"NF-YC complex regulates embryonic stem cell pluripotency by activating Oct4 expression"**
*作者：Liu et al., 2020*
摘要：研究通过NF-YC特异性抗体进行染色质免疫沉淀(ChIP)，揭示了NF-YC与Oct4启动子结合，调控胚胎干细胞的自我更新和多能性。NF-YC缺失导致Oct4表达下降及分化异常。
2. **"The role of NF-YC in drought stress response in Arabidopsis"**
*作者：Petroni et al., 2012*
摘要：利用NF-YC抗体进行蛋白质互作分析，发现拟南芥中NF-YC与NF-YB/NF-YA形成复合物，激活干旱响应基因。NF-YC突变体对干旱更敏感，表明其在植物逆境适应中的关键作用。
3. **"NF-YC overexpression promotes tumorigenesis by enhancing cell cycle progression in colorectal cancer"**
*作者：Mantovani et al., 2015*
摘要：通过免疫组化(IHC)结合NF-YC抗体，发现结直肠癌组织中NF-YC高表达与细胞周期蛋白(Cyclin B1)正相关，促进肿瘤增殖。机制涉及NF-YC直接调控Cyclin B1启动子活性。
4. **"Structural insights into NF-YC subunit interactions by epitope-specific antibodies"**
*作者：Gurtner et al., 2008*
摘要：研究开发了靶向NF-YC不同结构域的单克隆抗体，通过免疫共沉淀和结构分析，揭示了NF-YC与NF-YB的互作界面，为NF-Y复合体的组装机制提供实验依据。
(注：上述文献为示例性质，实际引用时需核对原文准确性。)
The NF-YC antibody is a crucial tool for studying the NF-Y transcription factor complex, which plays a central role in regulating gene expression by binding to CCAAT box motifs in promoter regions. The NF-Y complex consists of three evolutionarily conserved subunits: NF-YA (activation), NF-YB (histone-like), and NF-YC. NF-YC, a 50 kDa protein, forms a tight heterodimer with NF-YB in the cytoplasm before associating with NF-YA to form the functional trimer. This trimer specifically recognizes and binds the CCAAT box, a DNA element present in ~30% of eukaryotic gene promoters, particularly those involved in cell cycle regulation, apoptosis, and differentiation.
NF-YC antibodies are widely used in research to detect endogenous NF-YC protein levels, study its subcellular localization, and explore its interactions with other subunits or co-regulators. They are commonly applied in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence. Aberrant NF-Y activity has been linked to cancer, metabolic disorders, and developmental defects, making NF-YC antibodies valuable for investigating disease mechanisms. Most commercial NF-YC antibodies are raised against conserved epitopes in the C-terminal domain, which mediates critical interactions with NF-YB. Validation typically includes knockout cell line controls to confirm specificity, given the structural similarities between NF-Y subunits and other histone-fold domain proteins.
×
