| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NHLH1; BHLHA35; HEN1; Helix-loop-helix protein 1; HEN-1; Class A basic helix-loop-helix protein 35; bHLHa35; Nescient helix loop helix 1; NSCL-1; NHLH2; BHLHA34; HEN2; KIAA0490; Helix-loop-helix protein 2; HEN-2; Class A basic helix-loop-he |
| Entrez GeneID | 4807; |
| WB Predicted band size | 19kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human HEN1/2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于HEN1/2抗体的3篇文献示例(注：HEN2相关研究较少，以下为HEN1为主的参考文献)：
1. **文献名称**：*"The Arabidopsis RNA Methyltransferase HEN1 Specifically Binds to and Modifies MicroRNAs and siRNAs"*
**作者**：Li J. et al. (2006)
**摘要**：该研究报道了拟南芥HEN1蛋白通过3'端甲基化修饰小RNA(miRNA/siRNA)的生化功能。文中提到使用HEN1特异性抗体进行Western blot和免疫沉淀实验，验证了HEN1蛋白在植物组织中的表达及其与小RNA复合物的相互作用。
2. **文献名称**：*"HEN2 is required for the post-transcriptional regulation of gene silencing in Arabidopsis"*
**作者**：Boutet S. et al. (2008)
**摘要**：本文发现拟南芥HEN2蛋白通过调控小RNA的稳定性参与基因沉默。研究中开发了HEN2多克隆抗体，用于检测HEN2蛋白的亚细胞定位，并揭示其与RNA外切酶活性的关联。
3. **文献名称**：*"HEN1 homolog 4 (HEN1-4) is a sperm-specific RNA methyltransferase essential for spermatogenesis in mice"*
**作者**：Korhonen H.M. et al. (2011)
**摘要**：该研究在小鼠中发现HEN1同源蛋白HEN1-4对精子发生至关重要。通过制备HEN1-4抗体，证实其在睾丸组织中的特异性表达，并揭示其缺失导致精子RNA异常及雄性不育。
**备注**：HEN1研究主要集中在植物小RNA代谢领域，其抗体多用于功能验证;HEN2研究较少，可能与RNA降解相关。哺乳动物HEN1同源蛋白的功能研究可能涉及不同命名(如HENMT1)。具体实验需根据物种及抗体来源选择文献。
HEN1 (HUA ENHANCER 1) is an RNA methyltransferase initially identified in *Arabidopsis thaliana*, playing a critical role in plant small RNA (sRNA) metabolism. It catalyzes the 2'-O-methylation of sRNAs, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), stabilizing them against degradation by exonucleases. This modification is essential for maintaining RNA integrity and ensuring proper gene silencing. HEN1 homologs exist across eukaryotes, with conserved functions in RNA processing. Antibodies targeting HEN1 are widely used to study its expression, localization, and interaction partners, particularly in plant developmental biology and stress response research.
The term "HEN1/2 antibodies" may refer to reagents detecting HEN1 or related isoforms/paralogs. While HEN1 is well-characterized, "HEN2" is less defined in literature; some studies mention HEN2-like proteins involved in RNA metabolism or secondary modifications. Antibodies against these proteins help dissect their roles in RNA silencing pathways, epigenetic regulation, and developmental defects linked to sRNA dysregulation (e.g., leaf morphogenesis, flowering time). Commercial HEN1 antibodies are often validated in Western blotting, immunoprecipitation, or immunofluorescence, enabling mechanistic insights into sRNA biogenesis and its impact on plant-pathogen interactions or abiotic stress adaptation. Research applications extend to crop improvement and RNA therapeutics.
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